おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
rat
メーカー／製品名
Chemicon®
テクニック
immunohistochemistry: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
関連するカテゴリー
免疫原
FMRFamide
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
ホルモン及び受容体
ホルモン及び受容体
Anti-FMRFamide Antibody is an antibody against FMRFamide for use in IH.
Immunohistochemistry: 1:1,000 - 1:5,000
Application Notes for Immunohistochemistry:
- Suggested perfusion steps:
1) Ca2+-free Tyrode′s solution
2) Paraformaldehyde-picric acid fixative
3) 10% sucrose in PBS as a cryo-protectant
- Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
- Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature.
- Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C.
- Remove primary antibody and rinse 3 times in PBS.
- Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature.
Optimal working dilutions must be determined by the end user.
Application Notes for Immunohistochemistry:
- Suggested perfusion steps:
1) Ca2+-free Tyrode′s solution
2) Paraformaldehyde-picric acid fixative
3) 10% sucrose in PBS as a cryo-protectant
- Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
- Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature.
- Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C.
- Remove primary antibody and rinse 3 times in PBS.
- Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature.
Optimal working dilutions must be determined by the end user.
物理的形状
Serum
Liquid with 0.05% sodium azide.
保管および安定性
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB15348:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Tigran P Norekian et al.
The Journal of comparative neurology, 528(7), 1231-1254 (2019-11-22)
Cnidaria is the sister taxon to bilaterian animals, and therefore, represents a key reference lineage to understand early origins and evolution of the neural systems. The hydromedusa Aglantha digitale is arguably the best electrophysiologically studied jellyfish because of its system
Ryan E Hulett et al.
Proceedings. Biological sciences, 287(1931), 20201198-20201198 (2020-07-23)
The origin of bilateral symmetry, a major transition in animal evolution, coincided with the evolution of organized nervous systems that show regionalization along major body axes. Studies of Xenacoelomorpha, the likely outgroup lineage to all other animals with bilateral symmetry
Nada Abdellah et al.
Anatomical record (Hoboken, N.J. : 2007), 301(6), 1103-1114 (2018-01-19)
Neuropeptides AF (NPAF), FF (NPFF) and SF (NPSF) are RFamide neuropeptides known to be widely expressed in the mammalian central nervous system, where they fulfill a wide range of functions with pain modulation being the most prominent one. Recent evidence
Nagayasu Nakanishi et al.
Development (Cambridge, England), 139(2), 347-357 (2011-12-14)
As a sister group to Bilateria, Cnidaria is important for understanding early nervous system evolution. Here we examine neural development in the anthozoan cnidarian Nematostella vectensis in order to better understand whether similar developmental mechanisms are utilized to establish the
Ivan T Lee et al.
Nature communications, 11(1), 5453-5453 (2020-10-30)
The coronavirus SARS-CoV-2 is the causative agent of the ongoing severe acute respiratory disease pandemic COVID-19. Tissue and cellular tropism is one key to understanding the pathogenesis of SARS-CoV-2. We investigate the expression and subcellular localization of the SARS-CoV-2 receptor
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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