AB10910

Anti-Osteopontin Antibody

serum, from rabbit

• 別名: Bone sialoprotein 1, SPP1/CALPHA1 fusion, Urinary stone protein, bone sialoprotein I, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, e
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: mouse, human
• テクニック: immunohistochemistry: suitable; western blot: suitable
• NCBIアクセッション番号: NM_000582.2
• UniProtアクセッション番号: P10451
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SPP1(6696)

詳細

詳細

Osteopontin is a single-chain polypeptide. It is highly phosphorylated sialoprotein that is a prominent component of the mineralized extracellular matrices of bones and teeth. It is characterized by the presence of a polyaspartic acid sequence and sites of Ser/Thr phosphorylation that mediate hydroxyapatite binding, and a highly conserved RGD motif that mediates cell attachment/signaling. Expression of osteopontin in a variety of tissues indicates a multiplicity of functions that involve one or more of these conserved motifs. While the lack of a clear phenotype in osteopontin "knockout" mice has not established a definitive role for osteopontin in any tissue, recent studies have provided some novel and intriguing insights into the versatility of this enigmatic protein in diverse biological events, including developmental processes, wound healing, immunological responses, tumorigenesis, bone resorption, and calcification. The ability of osteopontin to stimulate cell activity through multiple receptors linked to several interactive signaling pathways can account for much of the functional diversity.

特異性

The antibody reacts with the 60 kDa osteopontin protein.

免疫原

Epitope: aa 75-90

Synthetic peptide corresponding to aa 75-90 of osteopontin protein.

アプリケーション

Research Category
細胞骨格

Detect Osteopontin using this Anti-Osteopontin Antibody validated for use in WB, IH.

Research Sub Category
Cytoskeleton

品質

Routinely evaluated by Western Blot on mouse kidney lysate.

Western Blot Analysis:
1:500 - 1:5,000 dilution of this antibody detected osteopontin in 10 μg of mouse kidney lysate.

ターゲットの説明

60 kDa

物理的形状

Serum containing 0.05% sodium azide.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Mouse kidney, COS-1 and human urine lysate

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1