おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
serum
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
mouse, human
化学種の反応性（ホモロジーによる予測）
rat (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
テクニック
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
ubiquitination (Lys119)
遺伝子情報
bovine ... H2Ax(531733)
dog ... H2Ax(489372)
human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)
詳細
H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins a damaged site. H2AX is phosphorylated soon after the appearance of double-stranded breaks. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.
特異性
This antibody recognizes Histone H2A.X when ubiquitylated at Lys119.
免疫原
Epitope: Lys119
Linear peptide containing ubiquitylated Lys119 of human Histone H2A.X.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Anti-Ubiquityl Histone H2A.X (Lys119) Antibody is a rabbit polyclonal antibody for detection of Ubiquityl Histone H2A.X (Lys119) also known as H2AXK119Ub, Histone H2A.X Ubiquityl K119) & has been validated in WB, IP, ICC.
Immunocytochemsitry Analysis: 1:500 dilution from a representative lot detected Histone H2A.X in RAW264.7 and NIH/3T3 cells.
Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.
Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.
品質
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.
ターゲットの説明
~ 23 kDa
物理的形状
Unpurified
Unpurified rabbit polyclonal containing serum with 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
アナリシスノート
Control
HeLa acid extract
HeLa acid extract
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
適切な製品が見つかりませんか。
製品選択ツール.をお試しください
保管分類コード
10 - Combustible liquids
WGK
WGK 1
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
AB10029:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Stéphanie Olivier-Van Stichelen et al.
Frontiers in genetics, 5, 256-256 (2014-08-20)
O-GlcNAc Transferase (OGT) catalyzes protein O-GlcNAcylation, an abundant and dynamic nuclear and cytosolic modification linked to epigenetic regulation of gene expression. The steady-state levels of O-GlcNAc are influenced by extracellular glucose concentrations suggesting that O-GlcNAcylation may serve as a metabolic
Yannick D Benoit et al.
Journal of cellular physiology, 228(4), 764-772 (2012-09-25)
Colorectal cancer is ranked among the top leading causes of cancer death in industrialized populations. Polycomb group proteins, including Suz12 and Ezh2, are epigenetic regulatory proteins that act as transcriptional repressors of many differentiation-associated genes and are overexpressed in a
Subhasree Roy Choudhury et al.
Carcinogenesis, 32(10), 1525-1532 (2011-07-30)
Polycomb group (PcG) protein-dependent histone methylation and ubiquitination drives chromatin compaction leading to reduced tumor suppressor expression and increased cancer cell survival. Green tea polyphenols and S-adenosylhomocysteine (AdoHcy) hydrolase inhibitors are important candidate chemopreventive agents. Previous studies indicate that (-)-epigallocatechin-3-gallate
Sivaprakasam Balasubramanian et al.
Cellular signalling, 27(7), 1336-1344 (2015-04-07)
The Bmi-1 Polycomb group (PcG) protein is an important epigenetic regulator of chromatin status. Elevated Bmi-1 expression is observed in skin cancer and contributes to cancer cell survival. (-) Epigallocatechin-3-gallate (EGCG), an important green tea-derived cancer prevention agent, reduces Bmi-1
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）