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詳細
The pCDF-1b plasmid features coexpression capabilities as well as the ability to express fusion proteins with a N-terminal His•Tag that results in native protein after purification and cleavage. This plasmid carries an origin derived from CloDF13 (1) and streptomycin/spectinomycin resistance, allowing for the option of coexpression with many other Novagen T7-based expression vectors.
The vector contains a T7 promoter, lac operator, ribosome binding site (rbs), an amino terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector-encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many of other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag coding sequence is compatible with purification, detection, and quantification (2).
The vector contains a T7 promoter, lac operator, ribosome binding site (rbs), an amino terminal His•Tag coding sequence, and multiple cloning site (MCS) regions designed to allow the generation of target proteins with minimal vector-encoded fusion. The Pml I cloning site allows direct fusion to the His•Tag sequence for inserts that are blunt and in the appropriate reading frame. For applications requiring a removable amino-terminal His•Tag sequence, the MCS includes a PshA I cloning site (GACNNNNGTC). The PshA I site overlaps the cleavage site for the enterokinase (EK) protease (AspAspAspAspLys). Cloning appropriately designed inserts into this site re-creates the full EK site and allows all amino-terminal vector-encoded sequences to be removed by EK digestion. The remainder of the MCS encodes restriction enzyme sites found in many of other Novagen expression vectors to facilitate insert transfer. An optional C-terminal S•Tag coding sequence is compatible with purification, detection, and quantification (2).
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
警告
Toxicity: Standard Handling (A)
その他情報
1. Nijkamp, H.J.J., De Lang, R., Stuitje, A.R., Van Den Elzen P.J.M., Veltkamp, E., and Van Putten, A.J. (1986) Plasmid16, 135–160.
2. Kim, J.S. and Raines, R.T. (1993) Protein Science2, 348–356.
2. Kim, J.S. and Raines, R.T. (1993) Protein Science2, 348–356.
法的情報
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
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Jan Code
71330-MG:
71330-10UG:
71330-3:
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