69182

DNase I, RNase free

For applications in which maintenance of RNA integrity is critical

• 別名: RNase-free DNase I
• UNSPSCコード: 12352202
• NACRES: NA.85

特性

• 形状: liquid
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• テクニック: DNA extraction: suitable
• 適合性: suitable for nucleic acid purification
• アプリケーション: diagnostic assay manufacturing
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

RNase-free DNase I digests either single- or double-stranded DNA, producing a mixture of mono- and oligonucleotides. Purified to be free of RNase, this preparation is qualified for applications in which maintenance of the integrity of RNA is critical. The enzyme selectively degrades DNA in the presence of RNA and can be used to remove DNA template following in vitro transcription reactions. This enzyme is also useful in other applications such as DNase footprinting and nick translation.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit will degrade 1 μg DNA in 10 minutes at 37°C. The reaction mixture (50 µl) contains 80 mM HEPES pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.

法的情報

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable