565764

γ-Secretase Substrate, Fluorogenic

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

• 別名: γ-Secretase Substrate, Fluorogenic, NMA-GGVVIATVK(DNP)- DR DR DR-NH₂
• 実験式（ヒル表記法）: C₇₀H₁₁₆N₂₆O₁₈
• 分子量: 1609.83
• UNSPSCコード: 12352202
• NACRES: NA.54

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 色: yellow
• 溶解性: DMSO: 5 mg/mL; 5% acetic acid: soluble
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence.

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.

生物化学的／生理学的作用

Cell permeable: no

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

NMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂

再構成

Following reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Farmery, M.R., et al. 2003. J. Biol. Chem.278, 24277.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable