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由来生物
human
品質水準
リコンビナント
expressed in E. coli
アッセイ
≥90% (SDS-PAGE)
形状
liquid
比活性
≥20 U/mg
分子量
37,400 g/mol
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
輸送温度
wet ice
保管温度
−70°C
遺伝子情報
human ... PTP1B(5770)
詳細
Research area: Cell Signaling
Recombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors. PTP1B comprises three domains: an N-terminal catalytic domain (1–300), a regulatory domain (301–400), and a C-terminal domain (401–435) that is accountable for directing the enzyme to the endoplasmic reticulum (ER) membrane.
Recombinant, human PTP1B expressed in E. coli. PTP1B, an ubiquitous nontransmembrane protein tyrosine phosphatase, was originally identified in human placenta. This highly active enzyme is useful for the study of tyrosine phosphatase kinetics, substrate specificity, and for screening inhibitors. PTP1B comprises three domains: an N-terminal catalytic domain (1–300), a regulatory domain (301–400), and a C-terminal domain (401–435) that is accountable for directing the enzyme to the endoplasmic reticulum (ER) membrane.
アプリケーション
Protein tyrosine phosphatase 1B (PTP1B), Human, Recombinant, E. coli has been used in the dephosphorylation assay of Glutathione S-transferase (GST) fusion proteins as well as in the in vitro kinase assays. It has also been used to construct a 66-member PTP1B library for discovery of enzyme inhibitors using click chemistry.
生物化学的／生理学的作用
Protein tyrosine phosphatase 1B (PTP1B) serves as a crucial enzyme in dephosphorylating insulin receptor and its downstream signaling components. Furthermore, it participates in the negative regulation of the leptin signaling pathway by dephosphorylation of the upstream signaling molecules, leading to the suppression of the neuropeptide Y synthesis, an appetite-stimulating hormone. Improper functioning of PTP1B has been associated with a variety of human diseases, including cancer, diabetes, obesity and inflammation.
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
単位の定義
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphopeptide substrate (Cat. No. 539737) per min at 30°C, pH 7.2 using 150 µM of substrate.
物理的形状
In 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.05% NP-40, pH 7.2.
再構成
Following initial thaw, aliquot and freeze (-70°C).
その他情報
Puius, Y.A., et al. 1997. Proc. Natl. Acad. Sci. USA94, 13420.
Liu, F., et al. 1996. J. Biol. Chem.271, 31290.
Liu, F., et al. 1996. J. Biol. Chem.271, 31290.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
539735-UG:
539735-50UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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