480025

NEMO-Binding Domain Binding Peptide, Cell-Permeable

A cell-permeable Antennapedia-NBD (wild type) fusion peptide that exhibits anti-inflammatory properties.

• 別名: NEMO-Binding Domain Binding Peptide, Cell-Permeable, DRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable
• 実験式（ヒル表記法）: C₁₇₀H₂₅₉N₄₉O₄₂S
• 分子量: 3693.24
• UNSPSCコード: 41116107

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic)
• 色: white
• 溶解性: DMSO: 50 mg/mL
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A cell-permeable Antennapedia-NBD (NEMO binding domain) (wild type) fusion peptide that exhibits anti-inflammatory activity in mouse model of acute inflammation. NBD is an amino terminal α-helical region of the NEMO (NF-κB essential modifier; IKKγ) associated with a carboxyl-terminal segment of IKKα and IKKβ. This peptide blocks the association of NEMO with the IKK complex and prevents NF-κB activation. NEMO is also reported to suppress the intrinsic basal activity of the IKK complex. Its anti-inflammatory properties are attributed to the inhibition of cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Does neither affect the TNF-α-induced phosphorylation of c-Jun nor the DNA binding of Oct-1 transcription factor.

A cell-permeable Antennapedia-NBD (wild type) fusion peptide that exhibits anti-inflammatory properties. Acts by inhibiting cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Suggested to block the interaction of NEMO with the IKK (IκB)-kinase complex and thereby prevents NF-κB activation. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.

生物化学的／生理学的作用

Cell permeable: yes

Primary Target
cytokine-induced NF-κB activation and NF-κB-dependent gene expression

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

H-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu-OH

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Jimi, E., et al. 2004. Nat. Med., 10, 617.
Siegmund, D., et al. 2001. J. Biol. Chem.276, 43708.
May, M.J., et al. 2000. Science289, 1550.
Li, Q., et al. 1999. Science284, 321.

Note: This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations. However, sufficient material will stay in the medium to permeate cells.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable