444256

MMP Substrate III, Fluorogenic

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination.

• 別名: MMP Substrate III, Fluorogenic, DABCYL-GABA-PQGL-E(EDANS)-AK-NH₂, TNO211
• 実験式（ヒル表記法）: C₆₃H₈₈N₁₆O₁₄S
• 分子量: 1325.54
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥97% (HPLC)
• 形状: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: red
• 溶解性: DMSO: soluble; Tris-buffered saline, pH 7.6: soluble
• 蛍光検出: λ_ex ~340 nm; λ_em ~485 nm
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (k_cat/K_m = 619,000 M^-1s^-1 for MMP-2, 206,000 M^-1s^-1 for MMP-9, 40,000 M^-1s^-1 for MMP-3, 21,000 M^-1s^-1 for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.

A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [k_cat/K_m = 619,000 M^-1s^-1 for MMP-2, 206,000 M^-1s^-1 for MMP-9, 40,000 M^-1s^-1 for MMP-3, and 21,000 M^-1s^-1 for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Detection of MMP activity

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH₂

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

その他情報

Beekman, B., et al. 1996. FEBS Lett390, 221.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable