440345-M

Lysozyme, Human Neutrophil

Occurs in specific granules of human polymorphonuclear leukocytes and takes part in antibacterial activity of leukocytes.

• 別名: Lysozyme, Human Neutrophil, N-Acetylmuramyl Hydrolase, Muramidase
• CAS番号: 9001-63-2
• Enzyme Commission number: 3.2.1.17 (BRENDA, IUBMB)
• MDL番号: MFCD00131557
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 詳細: Merck USA index - 14, 5640
• アッセイ: ≥95% (SDS-PAGE)
• フォーム: lyophilized
• 比活性: ≥30,000 Shugar units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 溶解性: aqueous buffer: soluble; water: soluble
• 保管温度: −20°C
• SMILES記法: N1([C@@H](CCC1)C(=O)N[C@@H](CCC(=O)N)C(=O)NCC(=O)N[C@@H]([C@H](CC)C)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc4c5c([nH]c4)cccc5)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc2c3c([nH]c2)cccc3)C(=O)N[C@@H](CCCN\C(=N\[H])\N)C(=O)N[C@@H]
• InChI: 1S/C99H159N37O23/c1-11-50(8)77(132-72(139)46-120-81(145)63(32-33-70(101)137)124-88(152)69-31-21-39-136(69)93(157)68(43-73(140)141)131-84(148)62(29-19-37-116-98(109)110)125-90(154)75(48(4)5)135-91(155)76(49(6)7)133-80(144)57(100)24-16-34-113-95(103)104)92(156)126-60(27-17-35-114-96(105)106)82(146)121-51(9)78(142)129-66(41-54-45-119-59-26-15-13-23-56(54)59)87(151)134-74(47(2)3)89(153)122-52(10)79(143)128-65(40-53-44-118-58-25-14-12-22-55(53)58)85(149)123-61(28-18-36-115-97(107)108)83(147)130-67(42-71(102)138)86(150)127-64(94(158)159)30-20-38-117-99(111)112/h12-15,22-23,25-26,44-45,47-52,57,60-69,74-77,118-119H,11,16-21,24,27-43,46,100H2,1-10H3,(H2,101,137)(H2,102,138)(H,120,145)(H,121,146)(H,122,153)(H,123,149)(H,124,152)(H,125,154)(H,126,156)(H,127,150)(H,128,143)(H,129,142)(H,130,147)(H,131,148)(H,132,139)(H,133,144)(H,134,151)(H,135,155)(H,140,141)(H,158,159)(H4,103,104,113)(H4,105,106,114)(H4,107,108,115)(H4,109,110,116)(H4,111,112,117)/t50-,51-,52-,57-,60-,61-,62-,63-,64-,65-,66-,67-,68-,69-,74-,75-,76-,77-/m0/s1
• InChI Key: ZJCXKXFAOCLSRV-RYLVIXIQSA-N

詳細

詳細

Native lysozyme from human neutrophils. Occurs in specific granules of human polymorphonuclear leukocytes and takes part in antibacterial activity of leukocytes. Hydrolyzes β1,4 linkages between N-acetylmuramic acid (2-acetamido-2-deoxy-3-O-[1′-carboxymethyl]-glucopyranose) residues in chitin.

警告

Toxicity: Standard Handling (A)

単位の定義

One Shugar unit is defined as the amount of enzyme that will cause a decrease in absorbance of 0.001 at 450 nm per min due to lysis of Micrococcus lysodeikticus at 37°C, pH 7.0.

物理的形状

Lyophilized from 100 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.

調製ノート

Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

再構成

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 days at 4°C or for up to 1 year at -20°C.

その他情報

Lollike, K., et al. 1995. Leukemia 9, 206.
Gordon, L.I., et al. 1979. J. Clin. Invest. 64, 226.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable