407707

PhosphoDetect Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb

liquid, Calbiochem^®

• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: liquid
• 含みます: ≤0.1% sodium azide as preservative
• 化学種の反応性: human
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• アイソタイプ: IgG
• 輸送温度: wet ice
• 保管温度: −70°C
• ターゲットの翻訳後修飾: phosphorylation (pTyr1162/pTyr1163)
• 遺伝子情報: human ... INSR(3643)

詳細

詳細

Note: 1 T = 1 test. Sufficient for 10 Western mini blots.

Rabbit polyclonal antibody adsorbed with non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the insulin receptor (IR) protein phosphorylated at Tyr^1162/1163 and insulin-like growth factor 1 receptor (IGF-1R) protein phosphorylated at Tyr^1162/1163.

Recognizes the ~95 kDa β subunit of the insulin receptor protein phosphorylated at Tyr¹¹⁶² and Tyr¹¹⁶³ in insulin receptor transfected cells. Cross-reacts with the insulin-like growth factor-1 receptor phosphorylated at Tyr¹¹³⁵ and Tyr¹¹³⁶.

This PhosphoDetect Anti-Insulin Receptor (pTyr^1162/1163) Rabbit pAb is validated for use in ELISA, WB, ICC for the detection of Insulin Receptor (pTyr^1162/1163).

免疫原

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^1162/1163 phosphorylation sites of human insulin receptor

アプリケーション

ELISA (see comments)

Immunoblotting (1:1000)

Immunocytochemistry (see comments)

警告

Toxicity: Standard Handling (A)

再構成

Following initial thaw, aliquot and freeze (-20°C).

その他情報

Bevan, P. 2001. J. Cell Sci.114, 1429.
Ottensmeyer, F.P., et al. 2000. Biochemistry39, 12103.
Virkamaki, A., et al. 1999. J. Clin. Invest.103, 931.
Schmid, E., at al. 1998. FASEB J.12, 863.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ebina, Y., et al. 1985. Cell40, 747.
Ganguly, S., et al. 1985. Current Topics in Cellular Regulation27, 83.
Grunberger, G., et al. 1984. Science223, 932.

The immunogen sequence is 100% conserved in the mouse and rat insulin recptor protein, but cross-reactivity has not been tested. This antibody has also been reported to work for ELISA and immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable