407615-M

Interleukin-1β, Human, Recombinant, E. coli

• 別名: Interleukin-1β, Human, Recombinant, E. coli, rhIL-1β
• CAS番号: 97599-21-8
• UNSPSCコード: 12352202

特性

• アッセイ: ≥97% (SDS-PAGE)
• 品質水準: 100
• フォーム: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: ≤1.0 EU/μg Endotoxin (EU/μg IL-1β)
• 保管温度: −70°C

詳細

詳細

Recombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. IL-1β is activated by proteolytic cleavage of the precursor molecule. Increases nitric oxide production in smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing. Induces nitric oxide synthase in insulin-producing cells. 1 µg of IL-1β corresponds to 1.9 x 10⁵ WHO units.

Recombinant, human interleukin-1β expressed in E. coli. Major form of interleukin-1 secreted by monocytes and macrophages. Induces nitric oxide synthase in pancreatic and smooth muscle cells and suppresses apoptosis in rat ovarian follicles. Involved in inflammatory and immune responses and promotes wound healing.

生物化学的／生理学的作用

ED₅₀ = <12 pg/ml as measured in a cell proliferation assay using the murine T-cell line, D10.G4.1 (Symons, J.A. et al. 1987)

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from sterile-filtered PBS, 50 µg BSA/µg IL-1β.

再構成

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.

Reconstitute to a concentration of ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

その他情報

Chun, S.Y., et al. 1995. Endocrinology136, 3120.
Kunz, D., et al. 1994. Biochem. J. 304, 337.
Eizirik, D.L., et al. 1993. FEBS Lett. 317, 62.
Dinarello, C>A> 1991. Blood77, 1627.
Casagli, M.C., et al. 1989. Biochem. Biophys. Res. Commun.162, 357.
Symons, J.A., et al. 1987. In Lymphokines and interferons, A Practical Approach (Clemens, M.J., et al. eds.) IRL Press, Oxford p. 272.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 13 - Non Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable