407294

αA-Interferon, Human, Recombinant, E. coli

• 別名: IFNA2A, IFNA2B, IFNA2C, rhIFN-αA
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 詳細: Merck USA index - 14, 4990
• 品質水準: 100
• アッセイ: ≥95% (SDS-PAGE)
• フォーム: liquid
• 比活性: ≥2.8 x 10⁸ units/mg protein; ≥5 x 10⁶ units/mL
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• テクニック: cell based assay: suitable
• NCBIアクセッション番号: NM_000605.4
• UniProtアクセッション番号: P01563
• 輸送温度: wet ice
• 保管温度: −70°C
• 遺伝子情報: human ... IFNA1(3340)

詳細

詳細

Research area: Immunoand cks

Interferons are cytokines secreted by mammalian cells. They are broadly divided into type I– interferon alpha (IFN-α) and interferon beta (IFN-β), type II – interferon-gamma (IFN-γ), and type III – interferon lambda (IFN-λ) based on their structure, function, and receptor specificity. Recombinant αA interferon is a hydrophobic protein belonging to the IFN-α family of proteins.

アプリケーション

Interferon alpha has been used to evaluate its effect on VSV (vesicular stomatitis virus) infection in pancreatic ductal adenocarcinoma (PDA) cell lines and study the relationship between type I IFNsignaling, resistance to VSV infection, and the JAK/STAT (Janus kinase/signal transducers and activators of transcription) signaling pathways in PDA cell lines. It has also been used to study the inhibitory effect of Morbillivirus V Proteins on Interferon Signaling Pathways.

生物化学的／生理学的作用

Recombinant, human αA-interferon expressed in E. coli have antiviral, antiproliferative, antineoplastic, and immunomodulating properties. Recombinant interferon αA (IFN-αA) is used as the first line of treatment for hepatitis C. It also displays a great therapeutic potential with chronic viral hepatitis, polycythemia, leukemia, and various other malignancies. It enhances natural killer cell activity, suppresses bone marrow function, and induces apoptotic cell death in non-melanoma skin cancer.

警告

Toxicity: Standard Handling (A)

単位の定義

One unit of interferon per ml of medium will cause a 50% inhibition of VSV-induced cytopathic effect on bovine MDBK cells. Units are determined with respect to the NIH international reference standard for human αA-IFN. Dilute in aqueous buffer containing at least 0.1% BSA or HSA.

物理的形状

In PBS containing 0.1% BSA.

調製ノート

Dilute only in buffers containing protein such as 0.1% BSA.

再構成

Following initial thaw, aliquot and freeze (-70°C).

その他情報

Fujishima, H., et al. 1998. Int. J. Cancer 76, 423.
Haria, M., and Benfield, P. 1995. Drugs 50, 873.
Familletti, P.C., et al. 1981. Methods Enzymol. 78, 387.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable