345749

GDF-8 Propeptide

≥90% (SDS-PAGE), lyophilized powder, mouse

• UNSPSCコード: 12352203
• NACRES: NA.26

特性

• product name: GDF-8 Propeptide, His•Tag^®, Mouse, Recombinant, GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.
• リコンビナント: expressed in mouse NSO cells (myeloma cells)
• 品質水準: 100
• アッセイ: ≥90% (SDS-PAGE)
• 形状: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze
• 不純物: ≤1.0 EU/μg protein Endotoxin
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Recombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.

アプリケーション

GDF-8 Propeptide, His•Tag, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

生物化学的／生理学的作用

ED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.

警告

Toxicity: Standard Handling (A)

物理的形状

Lyophilized from 0.2 µm filtered PBS, BSA.

再構成

Following reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

その他情報

Hill, J.J. et al. 2003. Molecular Endocrinology17, 1144.
Thies, R.S. et al. 2001. Growth Factors18, 251.
Chang, H. et al. 2002. Endocrine Reviews23, 787.
McPherron, A.C. et al. 1997. Nature387, 83.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2