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product name
Chromolith® RP-18 endcapped, L × I.D.50 mm x 4.6 mm HPLC column
物質
PEEK column
品質水準
認証
suitable for USP L1
製品種目
Chromolith®
特徴
endcapped
包装
1 ea of
標識化の程度
18% Carbon loading
パラメーター
200 bar pressure
45 °C max. temp.
テクニック
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
カラムL × 内径
5 cm × 4.6 mm
マトリックス
monolithic silica
マトリックス活性基
C18 bonding phase
ポアサイズ
13 nm mesoporosity
2 μm macroporosity
operating pH range
2-7.5
分離法
reversed phase
保管温度
no temp limit
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詳細
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® SpeedROD RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® SpeedROD RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® SpeedROD RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
Or
Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451]
Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Need more efficiency?
Chromolith® HighResolution RP-18 endcapped 50-4.6 HPLC columns [1.52021] provide a column efficiency exceeding 140,000 plates/meter.
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® SpeedROD RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® SpeedROD RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® SpeedROD RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
Or
Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451]
Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Need more efficiency?
Chromolith® HighResolution RP-18 endcapped 50-4.6 HPLC columns [1.52021] provide a column efficiency exceeding 140,000 plates/meter.
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
アプリケーション
- Simultaneous determination of eight arginine-related metabolites in cellular extracts using liquid chromatography-tandem mass spectrometry.: This study explores the usage of the Chromolith® RP-18 endcapped column for precise metabolic profiling, showcasing its effectiveness in analyzing arginine-related metabolites with high resolution and throughput (Amatya et al., 2020).
- Rapid Determination of 30 Polyphenols in Tongmai Formula, a Combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via Liquid Chromatography-Tandem Mass Spectrometry.: The use of Chromolith® RP-18 endcapped allowed for the fast and efficient separation of polyphenols in complex herbal formulations, illustrating its capabilities in herbal chemistry applications (Wang et al., 2017).
- Simultaneous Liquid Chromatographic Determination of Ebastine with Two Sympathomimetic Drugs Using a Monolithic Column.: This research highlights the Chromolith® RP-18 endcapped column′s ability to simultaneously analyze multiple compounds, thereby providing a method that improves the efficiency of pharmaceutical analyses (Ibrahim and Wahba, 2017).
アナリシスノート
Carbon content: 17.0 - 18.6 %
Pressure: ≤ 15 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.6 - 4.6
Tailing (USP): 0.90 - 1.55 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35
Pressure: ≤ 15 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.6 - 4.6
Tailing (USP): 0.90 - 1.55 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35
法的情報
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
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