14-736-M

TAO2 Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a MAP3K that doubly phosphorylates & activates the MAP2Ks, MEK3 & MEK6. For use in Kinase Assays.

• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

特性

• 由来生物: human
• 品質水準: 100
• リコンビナント: expressed in Sf21 cells
• アッセイ: 84% (Coomassie blue staining, SDS-PAGE)
• 比活性: 1562 U/mg
• 分子量: M_w 138.3 kDa
• メーカー／製品名: Chemicon^®
• テクニック: activity assay: suitable (kinase)
• NCBIアクセッション番号: NM_004783.2; NM_016151.2
• UniProtアクセッション番号: Q9UL54
• 遺伝子情報: human ... TAOK2(9344)

詳細

詳細

N-terminal 6His-tagged, recombinant, TAO2. TAO2 is a mitogen-activated protein kinase kinase kinase (MAP3K) that doubly phosphorylates and activates the MAP kinase kinases (MAP2Ks) MEK3 and MEK6.

Product Source: expressed in Sf21 cells

アプリケーション

Research Category
代謝

Research Sub Category
心血管疾患

代謝障害

生物化学的／生理学的作用

Protein Target: TAO2

Target Sub-Family: STE

品質

Routinely evaluated by phosphorylation of 0.8 mg/ml myelin basic protein (MBP). Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO2.

単位の定義

Specific Activity: where one unit of TOA2 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic per minute at 30°C with a final ATP concentration of 100 μM.

物理的形状

Ni²⁺/NTA-agarose

10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

保管および安定性

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable