14-475-M
Cdk2/Cyclin E Protein, active, 10 µg
Active complex of recombinant full length human Cdk2 containing a C-terminal His6-tag & recombinant full-length human Cyclin E containing an N-terminal GST-tag. For use in Kinase Assays.
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About This Item
UNSPSCコード:
12352202
eCl@ss:
32160405
NACRES:
NA.41
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由来生物
human
品質水準
リコンビナント
expressed in baculovirus infected Sf21 cells
シェルフライフ
1 yr
分子量
Mw 34 kDa (cdk2)
Mw 74 kDa (cyclin)
メーカー／製品名
Upstate®
テクニック
activity assay: suitable (kinase)
溶解性
water: soluble
NCBIアクセッション番号
UniProtアクセッション番号
遺伝子情報
human ... CCNE1(898) , CDK2(898)
詳細
Research area: APOPTOSIS
Complex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag. Product Source: Both are expressed by baculovirus in Sf21 insect cells The Cyclin E genes (CCNE1) gene located at 19q12 region in humans is responsible for encoding full-length protein of 410 amino acids.
Complex of recombinant full length human cdk2 containing a C-terminal His6-tag, and recombinant full-length human cyclin E containing an N-terminal GST-tag. Product Source: Both are expressed by baculovirus in Sf21 insect cells The Cyclin E genes (CCNE1) gene located at 19q12 region in humans is responsible for encoding full-length protein of 410 amino acids.
生物化学的／生理学的作用
Protein Target: CDK2/cyclinE
Target Sub-Family: CMGC
The Cyclin E1 protein binds to and activates Cyclin-dependent kinase 2 (CDK2), forming an active complex crucial for the initiation and progression of the S phase. Cyclin E/CDK2 complex primarily regulates cell cycle advancement and DNA replication by phosphorylating specific substrates. Elevated Cyclin E protein levels and heightened CDK2 kinase activity are both individually linked to unfavorable prognosis, decreased survival, and resistance to therapy in cancer patients. Overactivation of the Cyclin E/CDK2 complex directly disrupts DNA replication through multiple mechanisms, resulting in DNA double strand breaks (DSBs) and genomic instability. Targeting the oncogenic Cyclin E/CDK2 complex has emerged as a potential therapeutic approach against cancer.
Target Sub-Family: CMGC
The Cyclin E1 protein binds to and activates Cyclin-dependent kinase 2 (CDK2), forming an active complex crucial for the initiation and progression of the S phase. Cyclin E/CDK2 complex primarily regulates cell cycle advancement and DNA replication by phosphorylating specific substrates. Elevated Cyclin E protein levels and heightened CDK2 kinase activity are both individually linked to unfavorable prognosis, decreased survival, and resistance to therapy in cancer patients. Overactivation of the Cyclin E/CDK2 complex directly disrupts DNA replication through multiple mechanisms, resulting in DNA double strand breaks (DSBs) and genomic instability. Targeting the oncogenic Cyclin E/CDK2 complex has emerged as a potential therapeutic approach against cancer.
包装
Also available in 250μg size as catalog # 14-475M - Call for pricing and availability.
品質
routinely evaluated by phosphorylation of histone H1 by cdk2/cyclin E
物理的形状
Ni-NTA agarose
保管および安定性
1year at -70C
その他情報
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
試験成績書（COA）
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