1.12374

Thrombin

from bovine plasma, 50 NIH units/mg dry wt

• 別名: Thrombin, Coagulation factor IIa, Fibrinogenase
• CAS番号: 9002-04-4
• UNSPSCコード: 12352204
• EC Index Number: 232-648-7
• NACRES: NA.25

特性

• 由来生物: bovine plasma
• 品質水準: 200
• 形状: solid
• 比活性: 50 NIH units/mg dry wt
• 保管温度: 2-8°C

詳細

詳細

Thrombin, a glycosylated sodium-activated type II enzyme, is also called coagulation factor IIa. It contains two anion-binding exosites, ABE-I and ABE-II. Thrombin exists as α, β, and γ isoforms at different stages of its generation. Bovine a-thrombin consists of a light chain (A chain) and a heavy chain (B chain). These two chains are joined by one disulfide bond.

アプリケーション

Thrombin has been used in flow cytometry analysis to investigate the basal activation level of circulating platelets in mice. It has also been used to coagulate the plasma and allow the organotypic slice to adhere to the coverslip during culturing.

生物化学的／生理学的作用

Thrombin is a serine protease critical in the blood clotting process and activates clotting factors V, VIII, XI, and XII. It mediates the conversion of inactive plasma protein fibrinogen (factor I) into biologically active fibrin. Thrombin acts as a pro-coagulant and promotes platelet aggregation. It can also produce anti-coagulant effects. It is used to treat bleeding from capillaries and small venules. During vascular injury, thrombin is crucial for controlling the macrophages, neutrophils, monocytes, SMC, and dendritic cells.

アナリシスノート

Appearance (colour): almost white
Appearance (description): powder
Activity (fibrinogen; pH 7.6; 37 °C): ≥ 50 U/mg

安全性情報

• ピクトグラム: GHS07,GHS08
• シグナルワード: Danger
• 危険有害性情報: H315 - H319 - H334 - H335
• 注意書き: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable