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由来生物
rabbit
品質水準
結合体
ALEXA FLUOR™ 488
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
化学種の反応性
mouse, human
化学種の反応性（ホモロジーによる予測）
rat (based on 100% sequence homology), yeast (based on 100% sequence homology), chicken (based on 100% sequence homology)
テクニック
immunocytochemistry: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
dimethylation (Lys9)
遺伝子情報
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
詳細
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
特異性
Histone H3 dimethylated on lysine 9.
免疫原
Epitope: H3K9me2
KLH-conjugated, 2X-branched synthetic peptide containing the sequence ..RKme2STG.., in which Kme2 corresponds to dimethyl-lysine at residue 9 of human Histone H3.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
核受容体
核受容体
Anti-dimethyl-Histone H3 (Lys9) Antibody, AlexaFluor 488 Conjugate is an antibody against dimethyl-Histone H3 (Lys9) Antibody for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl-Histone H3 (Lys9) in HepG2 cells.
The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.
The unconjugated antibody (Cat. No. 07-441) is shown to be suitable also for Western blotting, dot blot, immunocytochemistry, and peptide inhibition assay (peptide blocking) applications.
品質
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected dimethyl-Histone H3 (Lys9) in HeLa cells.
ターゲットの説明
~17 kDa observed
物理的形状
Protein A purified
Purified rabbit antibody conjugate in PBS with 15 mg/ml BSA and 0.1 % sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
法的情報
ALEXA FLUOR is a trademark of Life Technologies
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
07-441-AF488:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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