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安全性情報

07-2167

Sigma-Aldrich

Anti-SUMO 2/3 Antibody

from rabbit, purified by affinity chromatography

別名:

SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae), Ubiquitin-like protein SMT3B, SMT3 homolog 2, SMT3 (suppressor of mif two 3, yeast) homolog 2, SMT3 suppressor of mif two 3 homolog 2 (yeast), small ubiquitin-related modifier 2, small ubiquitin-lik

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702

由来生物

rabbit

品質水準

抗体製品タイプ

primary antibodies

クローン

polyclonal

精製方法

affinity chromatography

化学種の反応性

pig, human, mouse, rat

化学種の反応性(ホモロジーによる予測)

bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), opossum (based on 100% sequence homology), chimpanzee (based on 100% sequence homology)

テクニック

immunoprecipitation (IP): suitable
western blot: suitable

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... SUMO2(6613)
mouse ... Sumo2(170930)
opossum ... Sumo2(123244757)
rat ... Sumo2(690244)

詳細

Small ubiquitin-related modifier 2 (UniProt P61956; also known as HSMT3, Sentrin-2, SMT3 homolog 2, Smt3B, SUMO-2, Ubiquitin-like protein SMT3B) and 3 (UniProt P55854; also known as SMT3 homolog 1, Smt3A, SUMO-3, Ubiquitin-like protein SMT3A) are encoded by the SUMO2 (also known as SMT3B, SMT3H2; Gene ID 6613) and SUMO3 (also known as SMT3A, SMT3H1; Gene ID 6612) genes in human. SUMOylation, protein post-translation modification by small ubiquitin-like modifier (SUMO), is a signaling event in many cellular processes. SUMO proteins are translated as immature precursors and subsequently converted to their mature forms through the activity of sentrin/SUMO-specific proteases (SENPs). SUMOylation is a reversible process. SUMO E1 activating enzyme, E2 conjugating enzyme, and E3 ligase mediate SUMOylation of substrate proteins, while SENPs are responsible for the de-SUMOylation. SUMOylation usually occurs at lysine residues in the consensus KxD/E motif, although not all such lysines become SUMOylated and SUMOylation can also occur on lysine residues outside of this motif. SUMO2 and 3 share 97% identity at the amino acid level, while SUMO1 shares only about 50% sequence homology with SUMO-2 and 3. In addition to difference in their target substrates, SUMO2/3 can be SUMOylated and form chains, whereas SUMO1 cannot and may serve as chain terminator. SUMO-2 can be covalently attached to proteins as a monomer or as a lysine-linked polymer. Its covalent attachment to its substrate via an isopeptide bond requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2, CBX4 or ZNF451. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Although SUMO-2 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system, however, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus (aa 94-95; propeptide) have been cleaved off.

特異性

This antibody recognizes SUMO 2/3 at the N-terminus.

免疫原

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the first 15 amino acids from the N-terminal region of human SUMO-2/3.

アプリケーション

Research Category
細胞シグナル伝達
Research Sub Category
ユビキチン及びユビキチン代謝
This SUMO 2/3 antibody is validated for use in WB & IP for the detection of the SUMO 2/3 protein.
Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected SUMO 2/3 on 10 µg of HeLa nuclear extract.
Immunoprecipitation Analysis: A previous was used by an independent laboratory in IP. (Li, T., et al. (2006). The Journal of Biological Chemistry. 281(47):36221-36227.)

品質

Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 1 µg/mL of this antibody detected SUMO 2/3 on 10 µg of HeLa nuclear extract.

ターゲットの説明

~12 kDa observed; 10.87 for SUMO-2 and 11.64 for SUMO-3 calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 without azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
HeLa nuclear extract

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

07-2167:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Nan Zhao et al.
Nature structural & molecular biology, 25(9), 885-893 (2018-09-05)
Viral infection perturbs host cells and can be used to uncover regulatory mechanisms controlling cellular responses and susceptibility to infections. Using cell biological, biochemical, and genetic tools, we reveal that influenza A virus (IAV) infection induces global transcriptional defects at

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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