07-1216

Anti-PP2A Antibody, Catalytic subunit

Upstate^®, from rabbit

• 別名: Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, delta isoform, Delta isoform of regulatory subunit B56, protein phosphatase, Protein phosphatase 2, regulatory subunit B (B56), delta isoform, Protein phosphatase 2, regulatory subunit B
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, chicken, bovine, rat, human
• メーカー／製品名: Upstate^®
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NM_006245
• UniProtアクセッション番号: Q14738
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPP2R5D(5528)

詳細

詳細

PP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism.

特異性

Peptide is highly conserved in human, rat, bovine, mouse and chicken.

Recognizes protein phosphatase 2A, catalytic subunit.

免疫原

Epitope: C-terminus

Synthetic peptide corresponding to a.a. 298-309 of the human sequence.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
キナーゼ及びホスファターゼ

Immunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as staining in normal human testis.

This Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.

品質

Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 μg of HEK293 lysates.

ターゲットの説明

60 kDa

関連事項

Replaces: AB1621

物理的形状

Affinity purified

Purified rabbit polyclonal IgG in buffer containing PBS with no preservatives.

保管および安定性

Stable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.

アナリシスノート

Control
HEK293 cell lysate

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable