06-659

Anti-phospho-c-Jun (Ser73) Antibody

Upstate^®, from rabbit

• 別名: Activator protein 1, Jun activation domain binding protein, Proto-oncogene c-jun, V-jun avian sarcoma virus 17 oncogene homolog, enhancer-binding protein AP1, jun oncogene, v-jun sarcoma virus 17 oncogene homolog, v-jun sarcoma virus 17 oncogene homolog
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 精製方法: affinity chromatography
• 化学種の反応性: mouse, human
• 化学種の反応性（ホモロジーによる予測）: Xenopus (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
• メーカー／製品名: Upstate^®
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_002219.1
• UniProtアクセッション番号: P05412
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer73)
• 遺伝子情報: human ... JUN(3725) ; mouse ... Jun(16476) ; rat ... Jun(24516) ; zebrafish ... Jun(335916)

詳細

詳細

c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced

signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.

特異性

This antibody detects phospho-c-Jun. May also detect phospho-JunD in properly treated cells since the sequence homology is identical (13/13).

免疫原

KLH-conjugated linear peptide corresponding to the human Phospho-C-Jun (Ser73) near the N-terminus.

アプリケーション

Anti-phospho-c-Jun (Ser73) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-c-Jun (Ser73) also known as Activator protein 1 & has been validated in ICC, IP & WB.

Western Blot Analysis :
A previous lot of this antibody detected 10 ng of phosphorylated gst-Jun, but did not detect 10 ng of nonphosphorylated Jun, as reported by an independent laboratory.

品質

Evaluated by Western Blotting in Anisomycin treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Phospho-C-Jun (Ser73) in 10 µg of Anisomycin treated NIH/3T3 cell lysate.

ターゲットの説明

~40 kDa

関連事項

Replaces: 04-213

物理的形状

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.

アナリシスノート

Control
Untreated and anisomycin-treated NIH/3T3 cell lysate.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1