07-024

Anti-gp91-phox Antibody

Upstate^®, from rabbit

• 別名: Cytochrome b(558) subunit beta, Cytochrome b558 subunit beta, Heme-binding membrane glycoprotein gp91phox, NADPH oxidase, Neutrophil cytochrome b 91 kDa polypeptide, Superoxide-generating NADPH oxidase heavy chain subunit, chronic granulomatous disease,
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human
• メーカー／製品名: Upstate^®
• テクニック: western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NM_000397
• UniProtアクセッション番号: P04839
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CYBB(1536)

詳細

詳細

Cytochrome b-245, beta polypeptide (chronic granulomatous disease), also known as CYBB and P91-PHOX, is a human gene encoding a glycoprotein.
Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.

特異性

Detects gp91-phox, Mr 75 kDa. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr 75 kDa, or appear as a smear between 75-91 kDa.

Predicted to cross react with mouse.

免疫原

KLH-conjugated, synthetic peptide corresponding to amino acids 548-560 of human gp91-phox (KQSISNSESGPRG). The immunizing sequence is identical in mouse, and it shares 11/13 sequence homology in bovine and 10/13 in porcine.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
酸化ストレス

Anti-gp91-phox Antibody is an antibody against gp91-phox for use in WB.

Immunoprecipitation:
Not recommended.

品質

Routinely evaluated by Western Blot on human neutrophil membranes.

Western Blot Analysis:
1-4 µg/mL of this lot detected gp91-phox in a HL60 membrane and RIPA cell lysate. A previous lot detected gp91-phox in human neutrophil membranes.

ターゲットの説明

Varies. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr ~75 kDa, or appear as a smear between 75-91 kDa

物理的形状

Protein A purified

Format: Purified

Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

保管および安定性

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

アナリシスノート

Control
Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1