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Key Documents

07-1224

Sigma-Aldrich

Anti-PP6C Antibody

from rabbit

別名:

H.sapiens mRNA for protein phosphatase 6, Serine/threonine-protein phosphatase 6, Protein phosphatase 6, catalytic subunit, Serine/threonine protein phosphatase catalytic subunit

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

polyclonal

化学種の反応性

mouse, rat, bovine, human

テクニック

immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

アイソタイプ

IgG

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... PPP6C(5537)

詳細

This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression.

特異性

Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa.

免疫原

The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH.

アプリケーション

Research Category
細胞シグナル伝達
Research Sub Category
キナーゼ及びホスファターゼ
Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP6 Polyclonal Antibody:
Human Testis
PP6 staining of round spermatids in normal human
testis, tissue pretreated with citrate buffer, pH 6.0.
Antibody was diluted 1:100, using IHC-Select
detection with HRP-DAB.
Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P).

品質

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 μg of Jurkat lysates.

ターゲットの説明

Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa

関連事項

Replaces: AB4091

物理的形状

Format: Purified
Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide.

保管および安定性

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
Jurkat Cell Lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

WGK 2

引火点(°F)

Not applicable

引火点(℃)

Not applicable


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Xiangli Cui et al.
Carcinogenesis, 31(10), 1734-1741 (2010-08-24)
We have recently shown that American ginseng (AG) prevents and treats mouse colitis. Because both mice and humans with chronic colitis have a high colon cancer risk, we tested the hypothesis that AG can be used to prevent colitis-driven colon
Jordan Wengrod et al.
Science signaling, 8(367), ra27-ra27 (2015-03-12)
Amino acid deprivation promotes the inhibition of the kinase complex mTORC1 (mammalian target of rapamycin complex 1) and activation of the kinase GCN2 (general control nonrepressed 2). Signaling pathways downstream of both kinases have been thought to independently induce autophagy.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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