06-519-AF488

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™ 488 conjugate

from rabbit, ALEXA FLUOR^™ 647

• 別名: Cyclic AMP-responsive element-binding protein 1 (pSer133), cAMP-responsive element-binding protein 1 (pSer133), CREB-1 (pSer133), CREB-A (pSer133), htCREB (pSer133), CREB-B (pSer119)
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 647
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 交差性: human, rat, mouse
• 交差性（ホモロジーによる予測）: Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology), chicken (based on 100% sequence homology), porcine (based on 100% sequence homology), guinea pig (based on 100% sequence homology)
• テクニック: immunofluorescence: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NP_604391
• UniProtアクセッション番号: P16220
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer133)
• 遺伝子情報: human ... CREB1(1385)

詳細

詳細

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation by various kinases, including PKA, and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

特異性

Recognizes phosphorylated CREB-A (pSer133), htCREB (pSer133), and CREB-B (pSer119) spliced isoforms reported by (UniProt P16220). May also recognize phosphorylated CREM and ATF-1 that contain homologous sequence to that of the immungen peptide.

免疫原

Epitope: pSer133.

KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB-A.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
転写因子

Anti-phospho-CREB (Ser133) Antibody, Alexa Fluor^™ 488 conjugate is an antibody against phospho-CREB for use in Immunofluorescence.

The unconjugated antibody (Cat. No. 06-519) is shown to be suitable also for Electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry, and Western blotting applications.

品質

Evaluated by Immunofluorescence in rat brain tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected phospho-CREB (Ser133) in rat brain tissue.

ターゲットの説明

~43 kDa observed.

物理的形状

Affinity purified

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable