05-443

Anti-VEGF Antibody, clone JH

clone JH, Upstate^®, from mouse

• 別名: Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100; 400
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: JH, monoclonal
• 化学種の反応性: rat, mouse, rabbit, human
• メーカー／製品名: Upstate^®
• テクニック: immunohistochemistry: suitable (paraffin)
• アイソタイプ: IgG1κ
• 適合性: not suitable for Western blot
• NCBIアクセッション番号: NM_003376
• UniProtアクセッション番号: P15692
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... VEGFA(7422)

詳細

詳細

The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.

特異性

Other species not tested.

Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).

免疫原

Recombinant human VEGF121. Clone JH121

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
成長因子及び受容体

Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.

Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.

This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.

品質

Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.

ターゲットの説明

34-54 kDa isoforms

物理的形状

Protein G Chromatography

Format: Purified

Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.

保管および安定性

Stable for 1 year at from date of receipt.

アナリシスノート

Control
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable