05-338

Anti-Fas Antibody (human, neutralizing), clone ZB4

clone ZB4, Upstate^®, from mouse

• 別名: APO-1 cell surface antigen, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, CD95 antigen, FASLG receptor, Fas (TNF receptor superfamily, member 6), Fas AMA, Fas antigen, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tu
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: ZB4, monoclonal
• 化学種の反応性: human
• メーカー／製品名: Upstate^®
• テクニック: flow cytometry: suitable; neutralization: suitable; western blot: suitable
• アイソタイプ: IgG1
• NCBIアクセッション番号: NM_152877
• UniProtアクセッション番号: P25445
• 輸送温度: dry ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FAS(355)

詳細

詳細

Fas (also known as Apo-1 and CD95) is a 40-50 kDa cell surface glycoprotein and a member of the TNF receptor family. Fas is an apotosis signalling molecule that forms the death inducing signalling complex (DISC) upon binding by the Fas ligand trimer on an adjacent cell. The Fas receptor also trimerizes, after which it is internalized and can then bind to the adaptor molecule FADD. FADD facilitates binding of caspase-8. which is activated to induce caspase-8 mediated apoptosis. Fas also plays a role in T cell mediated cytotoxicity and T cell development. Fas is found in the thymus, liver, heart, and ovary, where it is expressed on a minority of resting T and B cells and monocytes. The antagonistic ZB4 clone anti-Fas monoclonal has been used to block the apoptosis-inducing activity of the Anti-Fas CH-11 clone in multiple publications.

This product does not induce apoptosis in cell culture.

特異性

This antibody does not recognize TNF and mouse Fas.

This antibody recognizes the human cell surface antigen Fas expressed in various human cells, including myeloid cells, T lympho-blastoid cells and diploid fibroblasts. It does not induce apoptosis in cell culture.

免疫原

Recombinant human Fas.

アプリケーション

Research Category
アポトーシス及び癌

Research Sub Category
アポトーシス-追加

Anti-Fas Antibody (human, neutralizing), clone ZB4 detects level of Fas & has been published & validated for use in WB, FC, NEUT.

Flow Cytometry: A previous lot of this antibody used 5-20 μg/mL. Western Blot: A previous lot of this antibody used 5 μg/mL.

品質

Evaluated by neutralization of apoptosis induced by anti-Fas (human, activating), clone CH11 (Catalog # 05-201) treatment of human Jurkat cells.

Neutralization: 10-500 ng/mL of this antibody was incubated for one hour with human Fas-transfected cells and inhibited the anti-Fas (human, activating), clone CH11 (Catalog # 05-201). Cell viability was assessed using a WST-1 assay

ターゲットの説明

45 kDa

物理的形状

Protein A purified

Ammonium sulfate and purified mouse monoclonal IgG1 in PBS containing 50% glycerol. Store at -20°C.

Format: Purified

保管および安定性

Stable for 1 year at from date of receipt.

アナリシスノート

Control
Various human cells

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2