05-172-AF488

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate

clone Sm1.2, from mouse, ALEXA FLUOR^™ 488

• 別名: Insulin-like growth factor I, IGF-I, Mechano growth factor, MGF, Somatomedin-C
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.45

特性

• 由来生物: mouse
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 488
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: Sm1.2, monoclonal
• 化学種の反応性: chicken, rat, mouse, human, fish
• テクニック: immunocytochemistry: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000609
• UniProtアクセッション番号: P05019
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... IGF1(3479)

詳細

詳細

Insulin-like growth factor I (UniProt P05019; also known as IGF-I, Somatomedin-C) is encoded by the IGF1 (also known as IGFI, MGF) gene (Gene ID 3479) in human. Human insulin-like growth factor-I (IGF-I) is a single chain 70 amino acid polypeptide that shares structural homology with insulin and human IGF-II. IGF-I plays a critical role in growth, differentiation and development via autocrine, paracrine and endocrine mechanisms. It is a mitogen and also has effects on cell migration and differentiation. IGF-I is a regulator of metabolism and is also implicated in the pathophysiology of cancer, diabetes and obesity. Upon binding the tetrameric (alpha:beta)2 IGF-I receptor, the receptor alpha chains induce the tyrosine autophosphorylation of the beta chains, which in turn trigger cell type-specific downstream signaling cascade of events.

特異性

IGF-I (Russel, W.E., (1984). Proc. Natl. Acad. Sci. U. S. A. 81(8):2389-2392). 40% cross reactivity to IGF-II (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522).

免疫原

Highly purified human plasma insulin-like growth factor I (IGF-I; Somatomedin C; Sm-C) conjugated to mouse albumin (Van Wyk, J.J., and Bruton, E.T. (1997). Endocrinology. 138(10):4521-4522). Action of somatomedins on cell growth: Effect of selective neutralisation of somatomedin C (IGF-I) with a monoclonal antibody. In: Raiti, S., Tolman, and R.A. (eds.), Human Growth Hormone. pp. 585–599. Plenum Publishing Corp., New York, NY).

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
神経シグナリング

Anti-IGF-I Antibody, clone Sm1.2, Alexa Fluor^™ 488 Conjugate is an antibody against IGF-I for use in Immunocytochemistry.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected IGF-I in HeLa and NIH/3T3 cells.
The unconjugated antibody (Cat. No. 05-172) is shown to be suitable also for Western blotting, immunohistochemistry, immunoprecipitation, and neutralization applications.

品質

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected IGF-I in A431 cells.

ターゲットの説明

~17 kDa observed

物理的形状

Protein G Purified

Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable