05-163

Anti-PLCγ-1 Antibody

Upstate^®, from mouse

• 別名: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1, Phosphoinositide phospholipase C, Phospholipase C-gamma-1, monophosphatidylinositol phosphodiesterase, phosphatidylinositol phospholipase C, phosphoinositidase C, phospholipase C gamma 1,
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: B-2-5, monoclonal; B-20-3, monoclonal; B-6-4, monoclonal; D-7-3, monoclonal; E-9-4, monoclonal
• 化学種の反応性: mouse, human, bovine, rat, rabbit
• メーカー／製品名: Upstate^®
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NM_002660.2
• UniProtアクセッション番号: P19174
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PLCG1(5335)

詳細

詳細

Phospholipase C gamma 1 is a tyrosine kinase substrate for many receptor and nonreceptor tyrosine kinases. Activation of the enzyme produces two second messenger molecules, inositol 1,4,5-trisphosphate and diacylglycerol, which provoke the mobilization of intracellular Ca2+ and activation of protein kinase C. Although the mammalian genome encodes 10 known phosphoinositide specific PLCs, only the gamma 1 and gamma 2 isoforms are regulated by tyrosine kinase activity. PLC beta isoform activity is controlled by heterotrimeric G protein coupled receptors, while the mechanism of regulation of PLCgamma activity is unknown.

特異性

This antibody recognizes PLCγ-1, Mr 135 kDa. Reacts with some non-PLCγ-1 SH-3 containing proteins, including nck, Mr 47kDa (Park, D., 1992).

免疫原

Five individual clones raised against purified soluble phospholipase C (PLC)γ-1 from bovine brain.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
脂質シグナル伝達

Anti-PLCγ-1 Antibody detects level of PLCγ-1 & has been published & validated for use in IC, IP & WB.

Immunoprecipitation:
4 µg of a previous lot of antibody immunoprecipitated PLCγ-1 from non-stimulated A431 cell lysate, previous lots immunoprecipitated PLCγ-1 from rabbit brain cytosol preparation.

Immunocytochemistry:
5-10 µg/mL of previous lots of this antibody detected PLCγ-1 on ethanol-acetic acid [95:5] fixed A431 cells.

品質

Routinely evaluated in human A431 carcinoma cells, rabbit brain cytosol, and bovine brain cytosol preparations.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected PLCγ-1 in human A431 carcinoma cells; previous lots detected PKCγ-1 in rabbit brain cytosol and bovine brain cytosol preparations.

ターゲットの説明

135 kDa

物理的形状

Protein G Chromatography

Format: Purified

Liquid in 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide.
An equal mix of IgG antibodies produced by SP2/0-Ag14 x Balb/c spleen cells (Suh, P.G., 1988) and propagated as purified immunoglobulin. Clones B-2-5, B-6-4, B-20-3, D-7-3, E-9-4.

保管および安定性

Stable for 1 year at -20ºC from date of shipment.

アナリシスノート

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable