05-1320-M

Anti-EED Antibody, clone AA19

ascites fluid, from mouse

• 別名: WD protein associating with integrin cytoplasmic tails 1, embryonic ectoderm development
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: monoclonal
• 化学種の反応性: human, mouse
• テクニック: western blot: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_068676
• UniProtアクセッション番号: O75530
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... EED(8726) ; mouse ... Eed(13626)

詳細

詳細

Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis. EED is also known to bind H3K27me3 and can allosterically stimulate the methyltransferase activity of the PRC2 complex. (Ref.: Hojfeldt, JW. et al (2018) Nat. Struc. Mol. Biol. 25 (3); 225-232).

特異性

Not tested in other species.

This antibody recognizes mouse EED, (~30-65 kDa).

免疫原

Recombinant fusion protein of full length murine EED tagged with MBP.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

アポトーシス及び癌

Research Sub Category
エピジェネティクス

Anti-EED Antibody, clone AA19 is a mouse monoclonal antibody for detection of EED also known as WD protein associating with integrin cytoplasmic tails 1 ,embryonic ectoderm development & has been validated in WB.

品質

Routinely evaluated by Western Blot on A431cel lysatel.

Western Blot Analysis: 1:5,000 of this lot detected EED on 15 μg/mL of A431 cell lysate.

ターゲットの説明

Ranges from 30-65 kDa (5-6 isoforms of EED are detected).

物理的形状

Protein G Purified

Purified immunoglobulin in 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

アナリシスノート

Control
A431 Cell Lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable