05-1100X

Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2

clone Sulfo-1C-A2, Upstate^®, from mouse

• 別名: Clone Sulfo-1C-A2 Antibody, Sulfotyrosine Detection Antibody
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: culture supernatant
• 抗体製品タイプ: primary antibodies
• クローン: Sulfo-1C-A2, monoclonal
• 化学種の反応性: human, porcine, mouse, bovine
• 化学種の反応性（ホモロジーによる予測）: all
• メーカー／製品名: Upstate^®
• テクニック: ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2aκ
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Sulfotyrosine is a common post-translational modification of secretory, plasma membrane, and lysosomal proteins. The sulfation reaction is catalyzed by specific sulfotransferase enzymes in the trans-Golgi-network. Tyrosine sulfation occurs in all multicellular organisms and up to 1% of the tyrosines in the total protein content of a cell can be sulfated. Sulfotyrosines mediates many receptor-ligand binding interactions, including the interaction of P-selectin glycoprotein ligand-1 with P-selectin, factor VIII binding to von Willebrand factor, and interactions between thrombin and hirudin. Sulfotyrosine is also involved in the entry of several parasites and viruses; for example, tyrosine sulfation of the HIV-1 co-receptor CCR5 is required for viral entry into host cells, and tyrosine sulfation of the Duffy antigen/receptor for chemokines is crucial for erythrocyte invasion by the malaria parasite Plasmodium vivax.
This clone was derived from the antibody described in Kehoe et al., 2006.

特異性

Recognizes sulfated tyrosine residues on various proteins. Does not recognize phosphotyrosine or unmodified tyrosines.

The antibody is predicted to recognize any sulfotyrosine, regardless of surrounding amino acid sequence.

免疫原

Epitope: Sulfated Tyrosine

This antibody was developed using a phage display library as described in Kehoe et al., 2006. The selection antigens were KAKISDP-DY(SO3)MTGYMDAC and KDKKYATEY(SO3)-EYLDYDFC

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
一般的な翻訳後修飾

Anti-Sulfotyrosine Antibody, Clone Sulfo-1C-A2 is a high quality Mouse Monoclonal Antibody for the detection of Sulfotyrosine & has been validated in WB, IP, ELISA.

WB, IP, ELISA

品質

Assayed using sulfatase treated bovine fibrinogen vs. untreated fibrinogen.

ターゲットの説明

n/a

物理的形状

Culture supernatant, purified by protein G affinity chromatography

Format: Purified

Mouse IgG2aκ purified supernatant in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4 .

保管および安定性

Stable for 1 year from date of receipt when stored at +2-8°C.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable