04-1578

Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11

clone 10D11, from mouse

• 別名: Agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase, Bruton agammaglobulinemia tyrosine kinase, Bruton tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, tyrosine-protein kinase BTK
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: 10D11, monoclonal
• application: IP; WB
• 化学種の反応性: rat, human
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• citations: 1

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 10D11, monoclonal
• 化学種の反応性: rat, human
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000052.1
• UniProtアクセッション番号: Q06187
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... BTK(695)

詳細

詳細

Btk (Bruton’s tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. BTK plays a crucial role in B cell activation and development. Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner. Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA).

特異性

Recognizes human Btk, MW ~77 kDa.
A higher molecular weight protein may be detected, which is thought to be a phosphorylated species of Btk or a related protein.

免疫原

Epitope: Intracellular

GST-tagged recombinant protein corres-ponding to human Btk.

アプリケーション

Research Category
炎症及び免疫

Research Sub Category
免疫シグナル伝達

Detect Bruton′s tyrosine kinase (Btk) using this Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11 validated for use in WB & IP.

Immunoprecipitation Analysis: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Btk in the presence of 0.5% SDS. Not recommended for IP/kinase assays.

品質

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.05- 1 µg/mL of this antibody detected Btk on 10 µg of Raji cell lysate.

ターゲットの説明

~77 kDa

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Raji cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable