04-1475-I

Anti-CD27 Antibody, clone RM27-3E5

clone RM27-3E5, from rat

• 別名: CD27 antigen, CD27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: RM27-3E5, monoclonal
• application: FACS; Neutral
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; neutralization: suitable

特性

• 由来生物: rat
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: RM27-3E5, monoclonal
• 化学種の反応性: mouse
• テクニック: flow cytometry: suitable; neutralization: suitable
• アイソタイプ: IgG2aκ
• NCBIアクセッション番号: NP_001028298
• UniProtアクセッション番号: P41272
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Cd27(21940)

詳細

詳細

CD27 antigen (UniProt P41272; also known as CD27, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7) is encoded by the Cd27 (also known as Tnfrsf7) gene (Gene ID 21940) in murine species. CD27 is a TNF receptor superfamily member that binds to CD70 and mediates the survival, function, and differentiation of T-, B-, natural killer (NK), and plasma cells. Interaction with CD70 provides costimulation in T cells induces signaling events essential for cell proliferation, long-term maintenance of antigen-specific T cells, antiviral responses, antitumor immunity, and alloreactivity. CD27 ligation on B-cells by CD70 causes enhanced plasma cell formation and increased IgG production. CD27 is also used as a marker for memory B cells in the classification of B-cell deficiencies, including common variable immunodeficiency (CVID). CD27 is synthesized with a signal peptide (a.a. 1-23), which was removed posttranslationally to yield the mature type I transmembrane protein with an extracellular (a.a. 24-182) composed of three TNFR-Cys repeats (a.a. 26-63, 64-104, 105-141), followed by a transmembrane region (a.a. 183-203), and a cytoplasmic tail (a.a. 204-250).

特異性

Clone RM27-3E5 enhanced anti-CD3-stimulated T-cell proliferation when immobilized in vitro and augmented OVA-specific CTL induction when administrated in vivo (Roberts, D.J., et al. (2010). J. Immunother. 33(8): 769 779; Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).

免疫原

Epitope: extracellular domain

Human IgG1 Fc-tagged recombinant mouse CD27 extracellular domain (Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).

アプリケーション

Research Category
炎症及び免疫

Neurtralizing Analysis: A representative lot, when injected intraperitoneally following OVA-expressing B16 melanoma cells (B16-cOVA) implant, suppressed B16-cOVA lung metastasis and tumor growth among wild-type, but not Cd27-knockout, mice (Roberts, D.J., et al. (2010). J. Immunother. 33(8): 769 779).

Neurtralizing Analysis: A representative lot, when injected intraperitoneally during the induction phase of ragweed-induced experimental allergic conjunctivitis (EC), significantly affected the systemic immune responses, but not the severity of EC in mice (Sumi, T., et al. (2008). Immunol. Lett. 119(1-2):91-96).

This rat monoclonal Anti-CD27 Antibody, clone RM27-3E5, Cat. No. 04-1475-I is validated for use in Flow Cytometry and Neutralization applications for the analysis of CD27.

品質

Evaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 2 µL of this antibody detected CD27-positive mouse splenocytes.

ターゲットの説明

25.69/28.16 kDa (mature/pro-form) calculated.

関連事項

Replaces: 04-1475

物理的形状

Protein G purified.

Format: Purified

Purified rat IgG2a in PBS without preservatives.

保管および安定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable