907316

GlcA-ABP

95%

• 別名: (2S,3S,4S,5R,6S)-6-(2-(difluoromethyl)-4-(hex-5-ynamido)phenoxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-carboxylic acid, Glucuronic acid activity-based probe, Glucuronidated metabolite mimic
• 実験式（ヒル表記法）: C₁₉H₂₁F₂NO₈
• 分子量: 429.37
• UNSPSCコード: 12352101

特性

• アッセイ: 95%
• フォーム: solid
• 保管温度: −20°C

詳細

アプリケーション

This glucuronic acid activity-based probe (GlcA-ABP) is a glucuronidated metabolite mimic that provides in situ detection of active β-glucuronidases in the gut microbiome. The probe reacts with nucleophilic residues of the target, and the alkyne tag enables subsequent bioorthogonal tagging via click chemistry for enrichment (i.e. biotin tags) or imaging and cell sorting (i.e. fluorescent tags). With GlcA-ABP, Whidbey, et al were able to pair the activity-based probe (ABP) with fluorescence-activated cell sorting (FACS; ABP-FACS) to identify β-glucuronidase-active taxa in the gut microbiota.

Currently in gut microbiome research, access to and differentiation between subpopulations and their relationships to host biochemistry is a challenge. Functional and chemical approaches such as those with GlcA-ABP provide a complementary tool to gene sequencing.

その他情報

A Probe-Enabled Approach for the Selective Isolation and Characterization of Functionally Active Sub populations in the Gut Microbiome

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable