S8820
Protease Inhibitor Cocktail
SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use
Synonym(s):
Protease Inhibitor Tablets, protease inhibitor
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
product name
SIGMAFAST™ Protease Inhibitor Tablets, For General Use
biological source
bovine lung
bovine milk
Quality Level
form
tablet
solubility
water: 1 tablet/100mL, clear to hazy, colorless
storage temp.
2-8°C
Related Categories
General description
SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.
Specificity
Inhibits serine, cysteine, aspartic, and metalloproteases.
Application
SIGMAFAST Protease Inhibitor Tablets has been used:
- to wash raw semen for membrane protein analysis
- in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
- as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
- as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)
These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.
Features and Benefits
- Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
- Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
- Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
- Tested and optimized for general use: proven performance and reliability for a wide range of applications.
Components
AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Quantity
One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.
Physical form
Tablet that is water soluble.
Other Notes
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Legal Information
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
PRTR
Class I Designated Chemical Substances
JAN Code
S8820-2TAB:
S8820-VAR:
S8820-BULK:
S8820-20TAB:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Tran Hong Nha Nguyen et al.
Molecular & cellular proteomics : MCP, 11(11), 1140-1155 (2012-07-31)
Root hairs are single hair-forming cells on roots that function to increase root surface area, enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role as the site of infection by symbiotic nitrogen fixing rhizobia
Nihar J Mehta et al.
Free radical biology & medicine, 101, 44-52 (2016-11-05)
The dopamine oxidation product cysteinyl-dopamine has attracted attention as a contributor to the death of dopaminergic neurons in Parkinson's disease. Treatment of cysteinyl-dopamine with hypochlorite yields an even more cytotoxic product. This product has potent redox-cycling activity and initiates production
Jan Philipp Novotný et al.
PeerJ, 5, e3789-e3789 (2017-10-05)
Meidan Goldfinger et al.
European journal of immunology, 41(2), 491-502 (2011-01-27)
Plasma cells (PCs) secrete copious levels of immunoglobulins. To achieve this, their endoplasmic reticulum (ER) undergoes expansion in a process that requires continuous ER stress and activation of the unfolded protein response. It is important that protein synthesis, the driver
Artavazd Tadevosyan et al.
The Journal of biological chemistry, 285(29), 22338-22349 (2010-05-14)
Angiotensin-II (Ang-II) from extracardiac sources and intracardiac synthesis regulates cardiac homeostasis, with mitogenic and growth-promoting effects largely due to altered gene expression. Here, we assessed the possibility that angiotensin-1 (AT1R) or angiotensin-2 (AT2R) receptors on the nuclear envelope mediate effects
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service