Merck
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P9599

Sigma-Aldrich

Protease Inhibitor Cocktail

for plant cell and tissue extracts, DMSO solution

MDL number:
NACRES:
NA.77

Quality Level

form

DMSO solution

storage temp.

−20°C

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Specificity

Inhibits serine, cysteine, and aspartic proteases, metalloproteases, and aminopeptidases

Packaging

1, 5 mL in glass bottle

Application

Optimized and tested for use with plant tissue and cell extracts. Specific testing was done on plant seedling extracts from pea, bean, wheat, tobacco, and Arabidopsis, as well as leaf and root extracts from pea, wheat and tobacco.

Biochem/physiol Actions

This mixture contains individual components, including AEBSF, 1,10-Phenanthroline, Pepstatin A, Leupeptin, Bestatin, and E-64. Each component has specific inhibitory properties. AEBSF acts to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases. 1,10-Phenanthroline acts against metalloproteases.

Components

AEBSF
Bestatin
E-64
Leupeptin
Pepstatin A
1,10-Phenanthroline

Caution

The cocktail should be stored at -20°C, where it will retain stability for two years.

Quantity

One mL is recommended for the inhibition of proteases extracted from 30 g of plant tissue in a total volume of 100 ml.

Preparation Note

This product is supplied as a clear, faint pink solution in DMSO. One mL of solution is recommended for inhibition of protease activity in 100 mL of cell lysate from 30 g of various plant tissues or 10 g of baculovirus-infected cells. Extracts of plant seedlings from pea, bean, wheat, tobacco, and Arabidopsis have been tested. The roots of these plants have also been successfully tested.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

185.0 °F - closed cup

Flash Point(C)

85 °C - closed cup

Certificate of Analysis

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Certificate of Origin

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