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P0044

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 3

DMSO solution, for the inhibition of protein phosphatase 2A, alkaline phosphatases, protein phosphatases 1 and 2A, DMSO solution

Synonym(s):

Phosphatase inhibitor

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Phosphatase Inhibitor Cocktail 3, DMSO solution

form

DMSO solution

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Application

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.

Biochem/physiol Actions

This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.

Caution

As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.

Preparation Note

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

FSL

Group 4: Flammable liquids
Type 3 petroleums
Hazardous rank III
Water insoluble liquid

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

JAN Code

P0044-VAR:
P0044-5ML:4548173299174
P0044-BULK:
P0044-1ML:4548173299167
P0044-1ML-PW:
P0044-5ML-PW:

Certificates of Analysis (COA)

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Phosphatase Inhibitor Cocktail

Information on Phosphatase Inhibitor Cocktail for researchers and students from MilliporeSigma. Phosphorylation is a reversible protein post-translational modification.

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