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P0001

Sigma-Aldrich

ReadyShield® Phosphatase Inhibitor Cocktail

For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

Synonym(s):

Phosphatase inhibitor cocktail

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About This Item

UNSPSC Code:
12352200

Quality Level

200

form

ready-to-use solution

packaging

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

storage temp.

−20°C

Related Categories

General description

This phosphatase Inhibitor Cocktail contains inhibitors with a broad specificity for L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases. Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Specific for Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Deleterious substance

ISHL Indicated Name

Substances Subject to be Indicated Names

ISHL Notified Names

Substances Subject to be Notified Names

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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