Merck
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11836153001

Roche

cOmplete, Mini Protease Inhibitor Cocktail

Tablets provided in a glass vial

Synonym(s):
Protease inhibitor

form

tablet

usage

sufficient for 25 10mL extraction solution preparations

packaging

vial of 25 tablets

manufacturer/tradename

Roche

technique(s)

tissue processing: suitable

solubility

water: soluble

storage temp.

2-8°C

General description

cOmplete, Mini Protease Inhibitor Tablets, inhibit a broad spectrum of serine, cysteine, and metalloproteases, as well as calpains.
Due to the optimized composition of the tablets, they show excellent inhibition effects, and are well suited for the protection of proteins isolated from animal tissues, plants, yeast, and bacteria.
cOmplete, Mini Protease Inhibitor Tablets contain EDTA and both irreversible and reversible protease inhibitors.

Specificity

For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.

Application

cOmplete, Mini Protease Inhibitor Cocktail has been used as a
component of
  • lysis buffer for lysing glioma tumor samples
[87]
  • radioimmunoprecipitation (RIPA) protein extraction buffer for homogenization of heart tissues
  • [88]
  • resuspension buffer for retinal ganglion cells homogenization
  • [89]
    cOmplete, Mini, is used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.[02][03][04]
    cOmplete, Mini, contains both reversible and irreversible protease inhibitors. Therefore, we recommend the addition of cOmplete, Mini, to all stock buffers and solutions normally protected with protease inhibitors, and not  just during the initial purification steps.
    cOmplete, Mini contains EDTA (1 tablet yields a  1 mM EDTA solution  in 10 ml). Therefore, the extraction buffer should not contain divalent cations like Ca2+, Mg2+, or Mn2+, otherwise the inhibition of the metalloproteases might be incomplete. If the protein of interest will be purified by IMAC (Immobilized Metal  Affinity Chromatography), for example, Poly-His-tagged recombinant proteins, EDTA has to be eliminated (e.g., by dialysis) prior to the chromatography.
    Alternatively, the product cOmplete, Mini EDTA-free, can be used. These tablets are identical to cOmplete, Mini, with the exception that no EDTA or other chelating agent is  present.
    cOmplete, Mini Tablets, inhibit proteolytic activity in small extraction volumes (up to 10 ml). If you are working with a larger volume (up to 50 ml) we recommend to use a different cOmplete product. The composition of the cOmplete Tablet is identical to the cOmplete, Mini Tablet, therefore comparable results are achieved with both product types.

    Features and Benefits

    Use cOmplete, Mini Protease Inhibitor Tablets to protect your proteins from a wide range of proteases. In just minutes, uninhibited proteolytic activity can degrade the protein you have spent days isolating. Each of these convenient water-soluble tablets contains a blend of protease inhibitors that inhibits proteolytic activity from most cell types, including animals, plants, yeast, and bacteria. No weighing or measuring is necessary, ensuring consistent results.

    • Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
    • Complete protection: Instantly protect your proteins against a broad range of proteases.
    • Flexible: Protect proteins in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
    • Safe: Choose non-toxic inhibitors that pose no risk to you or those around you.
    • Suitable for IMAC purification: Purify poly-histidine tagged proteins with cOmplete His-Tag Purification Resin.

    Packaging

    Ships on Wet Ice in some cases however, shipping conditions may vary in certain regions.

    Components

    Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

    Quality

    Performance-tested with pancreas extract.

    Physical form

    Tablets in glass vials

    Preparation Note

    Working concentration: 1 tablet per 10 ml extraction solution
    Working solution: Preparation of Working Solution

    One cOmplete Mini Protease Inhibitor Tablet is sufficient for the inhibition of the proteolytic activity in 10 ml extraction solution. When very high proteolytic activity is present, one tablet should be used for 7 ml extraction buffer. Tablets can be added directly to the extraction medium. Alternatively, a stock solution (7 X conc.) can be prepared.

    Stock solution (7 X conc.)

    Dissolve one cOmplete Mini tablet in 1.5 ml dist. water or in 1.5 ml 100 mM phosphate buffer, pH 7.0.
    Storage conditions (working solution): The stock solution can be stored at 2 to 8 °C for 1 to 2 weeks, or at least 12 weeks at -15 to -25 °C.

    Reconstitution

    Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at 2 to 8 °C or at least 12 weeks at -15 to -25 °C; can be used in thiol-containing solutions at 15 to 25 °C.

    Other Notes

    For life science research only. Not for use in diagnostic procedures.

    Legal Information

    cOmplete is a trademark of Roche

    Pictograms

    Exclamation mark

    Signal Word

    Warning

    Hazard Statements

    Hazard Classifications

    Eye Irrit. 2 - Skin Irrit. 2

    Storage Class Code

    11 - Combustible Solids

    WGK

    WGK 2

    Flash Point(F)

    does not flash

    Flash Point(C)

    does not flash

    Certificate of Analysis

    Enter Lot Number to search for Certificate of Analysis (COA).

    Certificate of Origin

    Enter Lot Number to search for Certificate of Origin (COO).

    SDS

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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