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G5035

Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, recombinant, expressed in E. coli, suitable for cell culture

Synonym(s):

GM-CSF human

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About This Item

MDL number:
MFCD00166611
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE)

form

lyophilized powder

potency

≤0.1 ng/mL EC50

mol wt

14.6 kDa

packaging

pkg of 4X5 μg
pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

solubility

water: soluble 0.010 mL, clear, colorless

UniProt accession no.

P04141

storage temp.

−20°C

Gene Information

human ... CSF2(1437) , CSF2(1437)

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General description

Package size based on protein content

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Physical form

Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution.

Analysis Note

The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G5035-4X5UG:
G5035-5UG:
G5035-2UG-PW:
G5035-2UG:
G5035-5UG-PW:
G5035-VAR:
G5035-BULK:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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International journal of hematology, 100(4), 361-369 (2014-07-25)
Myelodysplastic syndrome (MDS) is a stem cell tumor characterized by dysplastic features and ineffective hematopoiesis in the early phase and leukemic progression in the late phase. Speculating that differences in the expression of genes and microRNA (miRNA) in control and
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Blood, 125(20), 3173-3182 (2015-03-15)
Because activating killer cell immunoglobulinlike receptors (KIRs) are heterogeneously expressed in the population, we investigated the role of donor activating KIRs in haploidentical hematopoietic transplants for acute leukemia. Transplants were grouped according to presence vs absence of KIR-ligand mismatches in
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PloS one, 9(9), e107063-e107063 (2014-09-10)
Cigarette smoking is a major pathogenic factor in lung cancer. Macrophages play an important role in host defense and adaptive immunity. These cells display diverse phenotypes for performing different functions. M2 type macrophages usually exhibit immunosuppressive and tumor-promoting characteristics. Although
T Kitamura et al.
Journal of cellular physiology, 140(2), 323-334 (1989-08-01)
We have established a novel cell line, designated as TF-1, from a patient with erythroleukemia, which showed complete growth dependency on granulocyte-macrophage colony-stimulating factor (GM-CSF) or on interleukin-3 (IL-3) and carried a homogeneous chromosomal abnormality (54X). Erythropoietin (EPO) also sustained
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