Skip to Content
Merck
All Photos(2)

Key Documents

Safety Information

M6518

Sigma-Aldrich

Macrophage Colony-Stimulating Factor human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

MCSF

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Macrophage Colony-Stimulating Factor human, M-CSF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

Quality Level

recombinant

expressed in E. coli

Assay

≥98% (SDS-PAGE and HPLC)

form

lyophilized powder

potency

0.500-5.000 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 37 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.000 EU/μg

color

white

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... CSF1(1435)

Looking for similar products? Visit Product Comparison Guide

General description

Macrophage Colony-Stimulating Factor (M-CSF) is a hematopoietic growth factor, which modulates the proliferation, differentiation and functional activation of monocytes. It is produced by fibroblasts, endothelial cells and bone marrow stromal cells. MCSF stimulates the functions of mature monocytes and macrophages.

Application

Macrophage Colony-Stimulating Factor human has been used for the differentiation of bone marrow-derived macrophages (BMDMs) and human monocyte-derived macrophages (MDMs). It has also been used for cell culture.

Biochem/physiol Actions

Macrophage colony-stimulating factor (M-CSF) augments many of the physiological functions of macrophages, and support osteoclast differentiation.

Other Notes

159 amino acids including N-terminal methionine (each monomer).

Physical form

Lyophilized from a sterile filtered buffered solution.

Analysis Note

The proliferative activity is tested in culture using the cytokine-dependent murine monocytic cell line M-NFS-60 cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

M6518-5UG:
M6518-VAR:
M6518-10UG:
M6518-1000UG:
M6518-BULK:
M6518-2UG:
M6518-10UG-PW:


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yuhao Liu et al.
Theranostics, 9(16), 4648-4662 (2019-08-02)
Rationale: Osteoporosis is a severe bone disorder that is a threat to our aging population. Excessive osteoclast formation and bone resorption lead to changes in trabecular bone volume and architecture, leaving the bones vulnerable to fracture. Therapeutic approaches of inhibiting
Avery's Diseases of the Newborn (2012)
Denise Buenrostro et al.
Bone, 113, 77-88 (2018-05-14)
The tumor-cell microenvironment is recognized as a dynamic place where critical cell interactions occur and play an important role in altering tumorigenesis. While many studies have investigated the effects of cellular cross-talk within distinct tumor microenvironments, these interactions have yet
MIR144 inhibits antimicrobial responses against Mycobacterium tuberculosis in human monocytes and macrophages by targeting the autophagy protein DRAM2
Kim JK, et al.
Autophagy, 13(2), 423-441 (2017)
Phenotypic and functional characterization of monoclonal antibodies with specificity for rhesus macaque CD200, CD200R and Mincle
Byrareddy SN, et al.
PLoS ONE, 10(10), e0140689-e0140689 (2015)

Articles

Read article on hematopoietic cytokines and hematopoiesis

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service