SRP3050

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): CSF-2, MGI-1GM, pluripoietin-a
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized
• potency: ≤0.1 ng/mL
• mol wt: 14.6 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P04141
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CSF2(1437)

Description

General description

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. Recombinant human GM-CSF is a 14.6kDa globular protein consisting of 128 amino acids containing two intramolecular disulfide bonds and two potential N-linked glycosylation sites. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

Application

Granulocyte-Macrophage Colony-Stimulating Factor human has been used in the medium for plating of the cells. It has been used in culture medium for the differentiation of human peripheral blood mononuclear cells.

Biochem/physiol Actions

GM-CSF (granulocyte-macrophage colony-stimulating factor, also referred to as CSF2) is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It enhances neutrophil migration and enhances the functional activity of the mature end-cells.

Sequence

MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 3.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable