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Safety Information

SRP3095

Sigma-Aldrich

IL-5 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

BCDFII, EDF

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.25 ng/mL ED50

mol wt

26.0 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

UniProt accession no.

P05113

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL5(3567)

Related Categories

General description

IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Produced by mast cells, T cells, and eosinophils, IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. Elevated levels of IL-5 lead to Eosinophilia, which may result in the induction of asthma and other allergic diseases. Human and murine IL-5 is cross-species reactive. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.

Biochem/physiol Actions

IL-5 is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils. Recombinant human IL-5 is a 26.0 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.

Sequence

MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

Physical form

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium glycinate, pH 8.5

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP3095-10UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Interleukin-5, eosinophils, and disease.
C J Sanderson
Blood, 79(12), 3101-3109 (1992-06-15)
Takehito Kobayashi et al.
Allergology international : official journal of the Japanese Society of Allergology, 64 Suppl, S30-S36 (2015-09-08)
Eosinophils recognize various stimuli, such as cytokines, chemokines, immunoglobulins, complement, and external pathogens, resulting in their accumulation in mucosal tissues and the progression of inflammation. Eosinophils are also involved in innate Th2-type immune responses mediated through endogenous danger signals, including
H D Campbell et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(19), 6629-6633 (1987-10-01)
The human eosinophil differentiation factor (EDF) gene was cloned from a genomic library in lambda phage EMBL3A by using a murine EDF cDNA clone as a probe. The DNA sequence of a 3.2-kilobase BamHI fragment spanning the gene was determined.
Ma Paz Zafra et al.
International journal of molecular sciences, 16(3), 5434-5451 (2015-03-13)
Eosinophils are one of the key inflammatory cells in asthma. Eosinophils can exert a wide variety of actions through expression and secretion of multiple molecules. Previously, we have demonstrated that eosinophils purified from peripheral blood from asthma patients express high

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