CG200

cGMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 96 assays
• Quality Level: 200
• technique(s): ELISA: suitable
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PRKG1(5592) , PRKG2(5593)

Description

Application

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.

Please refer to the attached Protocolfor details.

Kit Components Only

• 0.1 M Hydrochloride Acid 1 x 30
• Acetic Anhydride 1 x 1
• cGMP-Alkaline Phosphatase Conjugate 1 x 5
• cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5
• Cyclic GMP Standard 1 x 0.5
• Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea
• Neutralizing Reagent 1 x 6
p-Nitrophenyl Phosphate Substrate Solution 1 x 20
Plate Sealer 1 ea
Stop Solution 1 x 5
Triethylamine 1 x 2
Wash Buffer Concentrate 1 x 30

See All (12)

Safety Information

• Pictograms: GHS02,GHS06,GHS05
• Signal Word: Danger
• Hazard Statements: H226,H290,H302,H311,H314,H330,H335
• Precautionary Statements: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
• Target Organs: Respiratory system
• Supplementary Hazards: EUH071
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 120.2 °F
• Flash Point(C): 49 °C