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86R

Sigma-Aldrich

Leukocyte Alkaline Phosphatase Kit

based on naphthol AS-BI and fast red violet LB

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About This Item

UNSPSC Code:
12352106
eCl@ss:
42010105
NACRES:
NA.47

Quality Level

500

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

shipped in

wet ice

storage temp.

2-8°C

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Application

Leukocyte Alkaline Phosphatase (LAP) kits are intended for the qualitative demonstration of alkaline phosphatase activity in white blood cells.
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The film is then incubated in a mixture of naphthol AS-BI alkaline solution with fast red violet LB. The resulting insoluble diffuse, red dye deposit indicates sites of alkaline phosphatase activity.

Kit Components Only

Product No.
Description
  • Citrate Solution (915) 50 mL
  • FRV-Alkaline Solution (862) 10 mL
  • Hematoxylin Solution, Gill No. 3 (kit only) 50 mL
  • Naphthol AS-BI Alkaline Solution (861) 10 mL
  • Sodium Nitrite Solution (914) 10 mL

Signal Word

Danger

Hazard Statements

H290,H302,H315,H318,H373

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2 - STOT RE 2 Oral

Target Organs

Kidney

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

PDSCL

Please refer to KIT Component information

PRTR

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FSL

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ISHL Indicated Name

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ISHL Notified Names

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Cartagena Act

Please refer to KIT Component information

JAN Code

キットコンポーネントの情報を参照してください

Certificates of Analysis (COA)

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Alessandro Polini et al.
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R Raz et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(6), 2846-2851 (1999-03-17)
Propagation of mouse embryonic stem (ES) cells in vitro requires exogenous leukemia inhibitory factor (LIF) or related cytokines. Potential downstream effectors of the LIF signal in ES cells include kinases of the Src, Jak, and mitogen-activated protein families and the
Edgardo Rodríguez-Carballo et al.
PloS one, 9(7), e102032-e102032 (2014-07-10)
p38 MAPK activity plays an important role in several steps of the osteoblast lineage progression through activation of osteoblast-specific transcription factors and it is also essential for the acquisition of the osteoblast phenotype in early development. Although reports indicate p38
Salvatore Patanè et al.
Cancer research, 66(9), 4750-4757 (2006-05-03)
The MET oncogene was causally involved in the pathogenesis of a rare tumor, i.e., the papillary renal cell carcinoma, in which activating mutations, either germline or somatic, were identified. MET activating mutations are rarely found in other human tumors, whereas
Terence P Gade et al.
PloS one, 6(7), e22608-e22608 (2011-07-30)
The purpose of this study was to develop a paradigm for quantitative molecular imaging of bone cell activity. We hypothesized the feasibility of non-invasive imaging of the osteoblast enzyme alkaline phosphatase (ALP) using a small imaging molecule in combination with
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