91A

Leukocyte α-Naphthyl Acetate (Non-Specific Esterase) Kit

Kit formulated with all liquid reagents.

• MDL number: MFCD08705438
• UNSPSC Code: 41116123
• NACRES: NA.47

Properties

• Quality Level: 500
• form: liquid
• shelf life: Expiry date on the label.
• IVD: for in vitro diagnostic use
• application(s): hematology; histology
• shipped in: wet ice
• storage temp.: 2-8°C

Description

Application

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates. According to the methodology, α-naphthyl acetate is enzymatically hydrolyzed, liberating a free naphthol compound. This then couples with a diazonium compound, forming black colored deposits at sites of non-specific esterase activity. This enzyme is detected primarily in monocytes, macrophages and histocytes, and is normally absent in granulocytes. Lymphocytes may occasionally exhibit enzyme activity. A sodium fluoride inhibition procedure may be run to demonstrate monocyte inhibition. The procedure is run at 37 °C and may be utilized with blood, bone marrow films, tissue-touch preparations, cytocentrifuge preparations and frozen sections. The enzyme does not survive paraffin processing.

Kit Components Only

• TRIZMAL™ 7.6 Concentrate 50 mL
• Hematoxylin solution gill no. 3 50 mL

Kit Components Also Available Separately

• 916 α-Naphthyl Acetate Solution 10 mL
• 915 Citrate Solution, pH 3.6±0.1 (25 °C), 27 mM 50 mL
• 917 Fast Blue BB Base Solution 10 mL
• 914 Sodium nitrite solution 10 mL

Safety Information

• Pictograms: GHS02,GHS06,GHS08,GHS05
• Signal Word: Danger
• Hazard Statements: H226,H290,H301,H312 + H332,H319,H370
• Precautionary Statements: P210 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - STOT SE 1
• Target Organs: Eyes,Central nervous system
• Storage Class Code: 3 - Flammable liquids
• Flash Point(F): 98.1 °F
• Flash Point(C): 36.7 °C