391A

Leukocyte Peroxidase (Myeloperoxidase) Kit

DAB substrate kit

• MDL number: MFCD00131641
• UNSPSC Code: 12352106
• NACRES: NA.47

Properties

• usage: sufficient for 10-120 manual assays
• shelf life: Expiry date on the label.
• IVD: for in vitro diagnostic use
• application(s): hematology; histology
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Used to demonstrate polymorphonuclear cells in blood or bone marrow films. Each kit has sufficient reagents for 10 uses.

Application

Diaminobenzidine, as a benzidine substitute for myeloperoxidase, has less tinctorial power than benzidine. This made DAB much less attractive to histochemists. Hanker and associates improved DAB methodology. According to their modification, the brown reaction product is first intensified with copper salts and followed by application of Gill′s modified Papanicolaou stain, resulting in intense grey-black staining at sites of neutrophil and monocyte peroxidase.

The Leukocyte Peroxidase (Myeloperoxidase) kit is intended for use as a general laboratory histochemical stain for the demonstration of leukocyte peroxidase. This manual, qualitative procedure identifies leukocyte peroxidase in blood or bone marrow films of human specimens.

Kit Components Only

• Diaminobenzidine 10 vial(s)
• Copper nitrate 2 vial(s)
• Gill Modified EA solution 100 mL
• Glutaraldehyde solution 2 x 75
• Hematoxylin Solution, Gill No. 3 100 mL
• Scott's Tap Water Substitute Concentrate 100 mL
• TRIZMAL™ Buffer Concentrate 50 mL

Safety Information

• Pictograms: GHS02,GHS03,GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H225,H271,H290,H302 + H312 + H332,H314,H317,H334,H335,H341,H350,H360FD,H370,H373,H410
• Precautionary Statements: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Muta. 2 - Ox. Liq. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 Oral - STOT SE 1 - STOT SE 3
• Target Organs: Eyes,Central nervous system, Kidney, Respiratory system
• Supplementary Hazards: EUH071
• Storage Class Code: 5.1A - Strongly oxidizing hazardous materials
• WGK: WGK 3
• Flash Point(F): 57.2 °F
• Flash Point(C): 14 °C