Skip to Content
Merck
All Photos(3)

Key Documents

View All Documentation

Safety Information

C5499

Sigma-Aldrich

Cytochrome c Oxidase from bovine heart

greener alternative

5 mg protein/mL

Synonym(s):

EC 1.9.3.1, Ferrocytochrome-c:oxygen oxidoreductase, Complex IV

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

CAS Number:
9001-16-5
Enzyme Commission number:
1.9.3.1 (BRENDA, IUBMB)
EC Number:
232-586-0
MDL number:
MFCD00130899
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

bovine heart

Quality Level

200

form

buffered aqueous solution

specific activity

≥20 units/mg protein

mol wt

300-350 kDa by gel filtration

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

5 mg protein/mL

greener alternative category

, Enabling

shipped in

dry ice

storage temp.

−20°C

General description

Research area: Cell cycle

Cytochrome c oxidase contains 13 subunits, encoded by mitochondrial genes and nuclear genome. It is comprised of two heme groups (Fea and Fea3) and two copper centers, CuA and CuB.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell research. For more information see the article in biofiles.

Application

Cytochrome c Oxidase (CcO) from bovine heart has been used:
  • to study the effect of oxidized glutathione (GSSG) on the number of total free cysteines
  • in a histochemical assay to measure CcO activity in tissue sections
  • to study the redox reactions of cytochrome c in isolated mitochondria exposed to blue or red lasers using resonance Raman spectroscopy

Biochem/physiol Actions

Cytochrome c oxidase is the principal terminal oxidase of high oxygen affinity in the aerobic metabolism of all animals, plants, yeasts and some bacteria. It is present in the mitochondria of the more highly developed cells and in the cytoplasmic membrane of bacteria. Cytochrome c oxidase catalyses the electron transfer from cytochrome c to O2. This electron-transfer process produces a proton gradient across the membrane, which in turn drives the production of ATP. This enzyme is unique in providing energy for the cell bycoupling the electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Unit Definition

One unit of cytochrome c oxidase will oxidize 1.0 μmole of ferrocytochrome c per min. at 25 °C at pH 6.0.

Physical form

Supplied as a solution in 25 mM Tris-HCl buffer, pH 7.8, 5 mM EDTA, and 39 mM n-dodecyl β·-D-maltoside.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C5499-250UG:
C5499-BULK:
C5499-250UG-PW:
C5499-VAR:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Claudia R Oliva et al.
Oncotarget, 8(23), 37568-37583 (2017-04-30)
Patients with glioblastoma have one of the lowest overall survival rates among patients with cancer. Standard of care for patients with glioblastoma includes temozolomide and radiation therapy, yet 30% of patients do not respond to these treatments and nearly all
Zong-Heng Wang et al.
eLife, 8 (2019-10-16)
Oogenesis features an enormous increase in mitochondrial mass and mtDNA copy number, which are required to furnish mature eggs with an adequate supply of mitochondria and to curb the transmission of deleterious mtDNA variants. Quiescent in dividing germ cells, mtDNA
Claudia R Oliva et al.
The Journal of biological chemistry, 291(46), 24188-24199 (2016-09-30)
The enzyme cytochrome c oxidase (CcO) or complex IV (EC 1.9.3.1) is a large transmembrane protein complex that serves as the last enzyme in the respiratory electron transport chain of eukaryotic mitochondria. CcO promotes the switch from glycolytic to oxidative
Navjot Kaur et al.
Neuron, 107(6), 1197-1211 (2020-07-25)
Neural stem cells directly or indirectly generate all neurons and macroglial cells and guide migrating neurons by using a palisade-like scaffold made of their radial fibers. Here, we describe an unexpected role for the radial fiber scaffold in directing corticospinal
Matthew W Rice et al.
PloS one, 9(6), e100054-e100054 (2014-06-19)
Perturbations in metabolism are a well-documented but complex facet of schizophrenia pathology. Optimal cellular performance requires the proper functioning of the electron transport chain, which is constituted by four enzymes located within the inner membrane of mitochondria. These enzymes create

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service