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C2436

Sigma-Aldrich

Cytochrome c from Saccharomyces cerevisiae

≥85% based on Mol. Wt. 12,588 basis

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About This Item

CAS Number:
9007-43-6
EC Number:
232-700-9
MDL number:
MFCD00130890
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

Saccharomyces cerevisiae

Quality Level

200

Assay

≥85% based on Mol. Wt. 12,588 basis

form

powder

mol wt

12,588 Da

technique(s)

cell based assay: suitable

solubility

water: 10 mg/mL, clear, red to red-brown (dark red)

UniProt accession no.

P00044

application(s)

cell analysis

storage temp.

−20°C

Gene Information

Saccharomyces cerevisiae ... CYC1(853507)

General description

Cytochrome c (Cytc) comprises 104 amino acids and is a nuclear-encoded mitochondrial protein. This peripheral membrane protein is in spherical shape and has a molecular weight of 12,000 Da.

Application

Cytochrome c from Saccharomyces cerevisiae has been used in oocyte injection to induce caspase-3 activation and p38 MAPK (mitogen-activated protein kinase). It has also been used to analyze the aerobic reactions of nitric oxide.

Biochem/physiol Actions

Cytochrome c (Cytc) participates in mitochondrial electron transport and intrinsic type II apoptosis. It plays a key role in aerobic energy production. This multi-functional enzyme serves as a single electron carrier. Cytc helps in the formation of the apoptosome.
The ready fluctuation of cytochrome c within the cell between ferrous and ferric states, makes it an efficient biological electron-transporter. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal catalyst of respiration, it forms the essential electron-bridge between the respirable substrates and oxygen. Tracking cellular localization of cytochrome c in S. cerervisiae demonstrates that mitochondria are strongly involved in programmed cell death, a process very similar to apoptosis in higher organisms.

Quality

Care has been taken to maintain the native form of the protein. It has not been artificially oxidized or reduced during purification. Contains variable amounts of reduced cytochrome c.

Preparation Note

Prepared without using TCA.

Other Notes

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C2436-100MG:
C2436-BULK:
C2436-VAR:
C2436-250MG:
C2436-25MG:
C2436-5MG:

Certificates of Analysis (COA)

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Basic Properties of the p38 Signaling Pathway in Response to Hyperosmotic Shock
Messaoud NB, et al.
PLoS ONE, 10(9) (2015)
Reactions of nitric oxide with mitochondrial cytochrome c: a novel mechanism for the formation of nitroxyl anion and peroxynitrite
SHARPE MA and Cooper CE
The Biochemical Journal, 332(1), 9-19 (1998)
Membrane Proteins
The Membranes of Cells, 219-268 (2016)
The multiple functions of cytochrome c and their regulation in life and death decisions of the mammalian cell: from respiration to apoptosis
Huttemann M, et al.
Mitochondrion, 11(3), 369-369 (2011)
Ryota Yamasaki et al.
Biotechnology for biofuels, 11, 211-211 (2018-08-01)
We previously reversed methanogenesis in microbial fuel cells (MFCs) to produce electricity for the first time from methane by combining an engineered archaeal strain that produces methyl-coenzyme M reductase from unculturable anaerobic methanotrophs (to capture methane and secrete acetate) with
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