C7752

Cytochrome c from equine heart

≥95% based on Mol. Wt. 12,384 basis

• Synonym(s): Cytochrome c from horse heart
• CAS Number: 9007-43-6
• EC Number: 232-700-9
• MDL number: MFCD00130890
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: horse heart
• Assay: ≥95% (SDS-PAGE); ≥95% based on Mol. Wt. 12,384 basis
• form: powder
• mol wt: 12,384
• technique(s): cell culture | mammalian: suitable
• suitability: suitable for molecular biology
• UniProt accession no.: P00004
• storage temp.: −20°C
• Gene Information: horse ... CYCS(100053958)

Description

Application

Cytochrome c is used as an inducer of mitochondria-dependent apoptosis in various research studies. The identification and monitoring of oxidation sites in cytochrome c may have implications for assessing oxidative stress-related conditions and diseases. It could potentially be used as a biomarker to evaluate the extent of oxidative damage and the antioxidant capacity of cells and tissues.

Cytochrome c is suitable for use as a component of phosphate buffer for the detection and evaluation of complex IV activity using blue-native gel electrophoresis (BN-PAGE). It may also be used for the detection of extracellular superoxide anion (ECSA) in isolated kidney phagocytes.

The specific sites and extent of oxidation in horse cytochrome c treated with H₂O₂ and UV were characterized. It was suggested that the state of these sites could be used as a biomarker for the oxidative environment in a cell.

Biochem/physiol Actions

Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell.

Cytochrome c is primarily known as an electron-carrying mitochondrial protein. The transition of cytochrome c between the ferrous and ferric states within the cell makes it an efficient biological electron-transporter and it plays a vital role in cellular oxidations in both plants and animals. It is generally regarded as a universal catalyst of respiration, forming an essential electron-bridge between the respirable substrates and oxygen

Preparation Note

Prepared with acetic acid without using TCA.

Other Notes

View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves